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首页> 外文期刊>Chinese science bulletin >Calorimetric study on thermally-induced conformational change of dihydrofolate reductase
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Calorimetric study on thermally-induced conformational change of dihydrofolate reductase

机译：热诱导二氢叶酸还原酶构象变化的量热研究

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摘要

Dihydrofolate reductase (DHFR) (EC 1.5.1.3) exists in all biobodies It is an important component of the chemistry of DNA synthesis in cells. Methotrexate, a potent inhibitor of DHFR, is a well-known anticancer agent. It has been employed extensively in clinical treatment. Therefore, the study on the conformational change of DHFR gives occasion to people' s close attention. The thermally-induced conformational change of DHFR was investigated by differential scanning calorimetry (DSC) in the temperature range from 290 to 390 K. The experimental results showed that a large endothermic peak was observed at about 332.5 K when the enzyme lyophilized powder was heated at 10 K/min. When the sample was heated for the second time, the endothermic peak reappeared. It shows that the transition is reversible. After adding a certain amount of redistilled water, a flat peak was observed on DSC thermogram at about 313 K. The shapes and transition temperatures of the two peaks are obvionsly different. The thermodynamic parameters of thermally-induced conformation change of DHFR are reported in this note. Some experimental phenomena are discussed.
机译：二氢叶酸还原酶（DHFR）（EC 1.5.1.3）存在于所有生物体内，是细胞DNA合成化学中的重要组成部分。甲氨蝶呤是DHFR的有效抑制剂，是众所周知的抗癌药。它已被广泛用于临床治疗。因此，对DHFR构象变化的研究引起了人们的密切关注。通过差示扫描量热法（DSC）研究了DHFR的热诱导构象变化。在290至390 K的温度范围内。实验结果表明，将酶冻干的粉末在40℃加热时，在约332.5 K处观察到较大的吸热峰。 10 K /分钟当第二次加热样品时，吸热峰再次出现。它表明过渡是可逆的。加入一定量的蒸馏水后，在DSC温度谱图上大约313 K处观察到一个平坦的峰。两个峰的形状和转变温度明显不同。 DHFR的热诱导构象变化的热力学参数在此注释中报告。讨论了一些实验现象。

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