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首页> 外文期刊>Virology >Development of a novel mouse model for dengue virus infection.
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Development of a novel mouse model for dengue virus infection.

机译：登革热病毒感染的新型小鼠模型的发展。

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In the present study, we established an animal model for dengue virus infection using severe combined immunodeficient mice transplanted with a human hepatocarcinoma cell line (HepG2). At 7-8 weeks after transplantation, the HepG2-grafted mice were infected intraperitoneally with dengue virus type 2 (DEN-2). A higher titer of the virus was detected in the liver and serum but not in the brain in the early stage of postinfection. When the mice showed paralysis, the highest titer of virus was detected in the serum and brain. DEN-2 antigens were also found in HepG2 cells of the liver in the early stage and some neurons of the brain in the late stage. Upon clinical examination, thrombocytopenia, prolonged partial thromboplastin time, and increased hematocrit, blood urea nitrogen, and tumor necrosis factor alpha were seen in the paralyzed mice. Moreover, mild hemorrhage in the liver and tarry stool in the small intestine were observed in some mice. Our results show some similarities to human DEN infection and this mouse model might be valuable for studying some aspects of pathogenesis of this disease. Copyright 1999 Academic Press.
机译：在本研究中，我们建立了使用人肝癌细胞系（HepG2）移植的严重联合免疫缺陷小鼠的登革热病毒感染动物模型。移植后7-8周，移植了HepG2的小鼠腹膜内感染2型登革热病毒（DEN-2）。在感染后的早期，在肝脏和血清中检测到更高的病毒滴度，但在大脑中未检测到。当小鼠出现瘫痪时，在血清和大脑中检测到最高的病毒滴度。在早期肝的HepG2细胞和晚期脑的某些神经元中也发现了DEN-2抗原。经临床检查，在瘫痪的小鼠中发现了血小板减少症，部分凝血活酶时间延长和血细胞比容，血尿素氮和肿瘤坏死因子α增加。此外，在一些小鼠中观察到肝和小肠的柏油样便中轻度出血。我们的结果显示与人DEN感染有一些相似之处，并且该小鼠模型可能对于研究该疾病的发病机理具有重要意义。版权所有1999，学术出版社。

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