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首页> 外文期刊>Phytotherapy research: PTR >Antimicrobial Effects of Blueberry, Raspberry, and Strawberry Aqueous Extracts and their Effects on Virulence Gene Expression in Vibrio cholerae
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Antimicrobial Effects of Blueberry, Raspberry, and Strawberry Aqueous Extracts and their Effects on Virulence Gene Expression in Vibrio cholerae

机译：蓝莓，覆盆子和草莓水提物的抗菌作用及其对霍乱弧菌毒力基因表达的影响

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摘要

The antimicrobial effects of aqueous extracts of blueberry, raspberry, and strawberry on 13 pathogenic bacteria were evaluated. The minimum inhibitory concentrations and minimum bactericidal concentrations of the extracts were determined before and after neutralization to pH7.03 +/- 0.15. Both Gram-positive and Gram-negative pathogenic bacteria were selectively inhibited by the non-neutralized berries. Blueberry was the best inhibitor, and Vibrio and Listeria were the most sensitive bacteria. After neutralization, blueberry affected only Vibrio and Listeria, whereas the antimicrobial activities of raspberry and strawberry were abolished. The total contents of phenolics, flavonoids, and proanthocyanidins in the extracts were measured with colorimetric methods and were highest in strawberry, followed by raspberry, and then blueberry. We also studied the effects of sub-bactericidal concentrations of the three berry extracts on virulence gene expression in Vibrio cholerae. Real-time quantitative reverse transcription-polymerase chain reaction revealed that the three berry extracts effectively repressed the transcription of the tcpA gene. Raspberry also repressed the transcription of the ctxA gene, whereas blueberry and strawberry did not. However, the three berry extracts did not affect the transcription of toxT. These results suggest that the three berry extracts exert potent antimicrobial effects and inhibit the expression of the virulence factors of V. cholerae. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：评估了蓝莓，覆盆子和草莓的水提物对13种病原菌的抗菌作用。在中和至pH7.03 +/- 0.15之前和之后确定提取物的最小抑菌浓度和最小杀菌浓度。革兰氏阳性和革兰氏阴性致病菌均被未中和的浆果选择性抑制。蓝莓是最好的抑制剂，弧菌和李斯特菌是最敏感的细菌。中和后，蓝莓仅影响弧菌和李斯特菌，而覆盆子和草莓的抗菌活性被取消。用比色法测量提取物中酚类，类黄酮和原花青素的总含量，在草莓中含量最高，其次是覆盆子，然后是蓝莓。我们还研究了三种浆果提取物的亚杀菌浓度对霍乱弧菌毒力基因表达的影响。实时定量逆转录-聚合酶链反应显示，这三种浆果提取物有效地抑制了tcpA基因的转录。树莓还抑制了ctxA基因的转录，而蓝莓和草莓则没有。但是，这三种浆果提取物并未影响toxT的转录。这些结果表明，三种浆果提取物发挥了有效的抗菌作用，并抑制了霍乱弧菌的毒力因子的表达。版权所有（C）2015 John Wiley＆Sons，Ltd.

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