...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World journal of gastroenterology : >Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections
【24h】

Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections

机译：铁调素表达的分析：石蜡切片的原位杂交和定量聚合酶链反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. RESULTS: Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. CONCLUSION: We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC.
机译：目的：建立使用从石蜡包埋切片中分离的RNA和肝细胞癌（HCC）的原位杂交技术对铁调素进行定量聚合酶链反应（PCR）的方法。方法：从68例肝癌石蜡包埋样品中分离出石蜡包埋切片的总RNA。样本来自54位男性和14位女性患者，平均年龄为66.8±7.8岁。进行定量PCR。还对hepcidin进行了免疫组织化学和原位杂交。结果：使用从肝癌石蜡包埋切片中分离的RNA进行了hepcidin的定量PCR。 Hepcidin mRNA在癌组织中的表达水平明显高于非癌组织。成功建立了铁调素原位杂交的方法，这表明铁调素mRNA在非癌组织中表达，而在癌组织中不存在。结论：我们建立了新的方法，使用从石蜡包埋的切片中分离的RNA和肝细胞癌的原位杂交技术定量检测铁调素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号