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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Cytotoxic, immunosuppressive and trypanocidal activities of agrocybin, a polyacetylene produced by Agrocybe perfecta (Basidiomycota)
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Cytotoxic, immunosuppressive and trypanocidal activities of agrocybin, a polyacetylene produced by Agrocybe perfecta (Basidiomycota)

机译：农杆菌肽的细胞毒性，免疫抑制和锥虫活性，农杆菌肽由完美农杆菌（Basidiomycota）生产

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摘要

The ethyl acetate extract from the culture of the fungus Agrocybe perfecta (Rick) Singer was selected for further study in a screening of Brazilian basidiomycetes for bioactivity. The extract showed significant activity against the recombinant enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, lymphocyte proliferation in human peripheral mononuclear cells (PBMC) stimulated with phytohemaglutinin (PHA), and the human cancer cell lines UACC-62 (melanoma), MCF-7 (mammary), and TK-10 (kidney). The chromatographic fractionation of the extract was monitored by the above bioassays and showed that agrocybin was the active component. Agrocybin, a known polyacetylene amide, showed an IC of 2 oM in the TryR assay but killed only 60% of the trypomastigote form of T. cruzi in infected murine blood even at 680 oM. This weaker activity could be due to the low temperature used to mimic banked blood or as a consequence of its inactivation by blood, already reported in the literature. On the other hand, it inhibited the proliferation of PBMC by 50% at 3.4 oM and the growth of the cancer cell lines at concentrations between 9 and 24.5 oM. Measurements of DNA fragmentation using flow cytometry suggest that agrocybin promotes cell death via apoptosis.
机译：选择来自农杆菌（Rick）Singer真菌培养物中的乙酸乙酯提取物，以进一步筛选巴西担子菌的生物活性。提取物显示出对克氏锥虫重组酶锥虫硫还原酶（TryR），植物血凝素（PHA）刺激的人外周血单个核细胞（PBMC）中的淋巴细胞增殖以及人癌细胞UACC-62（黑素瘤），MCF- 7（乳腺）和TK-10（肾脏）。通过上述生物测定法对提取物的色谱分离进行监测，结果表明农杆菌肽是活性成分。 Agrocybin（一种已知的聚乙炔酰胺）在TryR分析中的IC为2 oM，但即使在680 oM的浓度下，也只能杀死60％的被感染鼠科动物的克鲁索锥虫形式。这种较弱的活性可能是由于用于模拟堆积的血液的低温或由于其已被血液灭活所致，已在文献中进行了报道。另一方面，它在3.4 oM时抑制PBMC的增殖50％，并在9至24.5 oM的浓度下抑制癌细胞系的生长。使用流式细胞仪对DNA片段的测量表明，土壤杆菌素可通过凋亡促进细胞死亡。

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