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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Molecular cloning, genetic organization of gene cluster encoding phenol hydroxylase and catechol 2,3-dioxygenase in Alcaligenes faecalis IS-46
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Molecular cloning, genetic organization of gene cluster encoding phenol hydroxylase and catechol 2,3-dioxygenase in Alcaligenes faecalis IS-46

机译：粪产碱菌IS-46中编码酚羟化酶和儿茶酚2,3-二加氧酶的基因簇的分子克隆，遗传组织

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摘要

Alcaligenes faecalis IS-46 can utilize phenol as the sole carbon and energy source at concentration up to 1000 mg/l. In this report we created a cosmid library of this strain and the two clones specifying the whole L-46d type of phenol hydroxylase gene cluster were identified and characterized. Sequence analysis revealed that although the overall gene organization of the clusters was quite similar, few coding sequences differed or were found to have two copies compared with other source organisms. One of these coding sequences showed a good protein sequence similarity to a hypothetical protein and one matched with a regulatory protein of the LysR system. Their putative role in phenol degradation was discussed. Bioinformatic analysis suggested tentative phylogenetic assignments of the retrieved clusters. This work described for first time the complete nucleotide sequence and genetic organization of the whole phenol hydroxylase gene cluster in A. faecalis species.
机译：粪产碱杆菌IS-46可以利用苯酚作为唯一的碳和能源，浓度最高可达1000 mg / l。在本报告中，我们创建了该菌株的粘粒文库，并鉴定和鉴定了两个指定整个L-46d型酚羟化酶基因簇的克隆。序列分析显示，尽管簇的总体基因组织非常相似，但与其他来源生物相比，几乎没有编码序列差异或发现有两个拷贝。这些编码序列之一显示出与假设蛋白质的良好蛋白质序列相似性，并且一个与LysR系统的调节蛋白匹配。讨论了它们在苯酚降解中的假定作用。生物信息学分析建议对检索到的簇进行初步的系统发育分配。这项工作首次描述了粪屎曲霉物种中整个酚羟化酶基因簇的完整核苷酸序列和遗传组织。

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