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首页> 外文期刊>World Journal of Microbiology & Biotechnology >A quick screening method to identify beta-glucosidase activity in native wine yeast strains: application of Esculin Glycerol Agar (EGA) medium.
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A quick screening method to identify beta-glucosidase activity in native wine yeast strains: application of Esculin Glycerol Agar (EGA) medium.

机译：一种快速筛选天然酒酵母菌株中β-葡萄糖苷酶活性的方法：应用Esculin甘油琼脂（EGA）培养基。

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摘要

One hundred and fifty-four yeast strains were isolated from grapes and musts of Uruguayan vineyards and wineries. Only thirty strains showed beta-glucosidase activity in Esculin Glycerol Agar (EGA) solid medium. Twenty-one were non-Saccharomyces and nine were Saccharomyces cerevisiae strains. The objective of this study was to evaluate the suitability of Esculin Glycerol Agar (EGA) solid medium for screening beta-glucosidase activity in native yeasts strains. Halo sizes measured in the EGA solid medium were correlated to the Glycosyl-Glucose (GG) indexes measured after fermentation of grape musts with each strain. The two S. cerevisiae strains with the best performance were selected for further fermentations on a Muscat Miel grape must, rich in bound monoterpenes. The levels of free linalool, hodiol I and geraniol increased significantly as compared to fermentation with a commercial wine yeast strain. These results show the suitability of this simple and economic medium to identify S. cerevisiae glucosidase producers with a potential impact on real winemaking conditions. On the other hand, great variability was found for the non-Saccharomyces strains, and this would demand further studies for each species. In conclusion, the use of EGA solid medium shows that the screening method is suitable for exploring the glucosidase activity of native strains of S. cerevisiae and shows good correlation with its real impact on free aroma compounds in the final wine.
机译：从乌拉圭葡萄园和酿酒厂的葡萄和葡萄汁中分离出了一百四十四种酵母菌株。在Esculin甘油琼脂（EGA）固体培养基中，只有三十个菌株显示出β-葡萄糖苷酶活性。 21个是非酿酒酵母菌株，而9个是酿酒酵母菌株。这项研究的目的是评估Esculin甘油琼脂（EGA）固体培养基在天然酵母菌株中筛选β-葡萄糖苷酶活性的适用性。在EGA固体培养基中测得的光环大小与葡萄汁在每种菌株发酵后测得的糖基葡萄糖（GG）指数相关。两个 S。选择具有最佳性能的啤酒酵母菌株在富含结合的单萜的马斯喀特米尔葡萄葡萄汁上进一步发酵。与市售葡萄酒酵母菌株发酵相比，游离芳樟醇，hodiol I和香叶醇的含量显着增加。这些结果表明，这种简单且经济的媒介适合识别S。酿酒酵母葡糖苷酶生产者，可能会对真实的酿酒条件产生影响。另一方面，非酵母属菌株的变异性很大，这需要对每个物种进行进一步的研究。总之，使用EGA固体培养基表明，该筛选方法适用于探索天然iS菌株的葡糖苷酶活性。酿酒酵母，并与其对最终葡萄酒中游离香气化合物的实际影响显示出良好的相关性。

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