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首页> 外文期刊>Chinese science bulletin >Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequence tags
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Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequence tags

机译：大豆疫霉表达序列标签衍生的简单序列重复标记的分析

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摘要

Five thousand and eight hundred publicly available expressed sequence tags (ESTs) of Phytophthora sojae were electronically searched and 415 simple sequence repeats (SSRs) were identified in 369 ESTs. The average density of SSRs was one SSR per 8.9 kbof EST sequence screened. The most frequent repeats were trinucleotide repeats (50.1%) and the least frequent were tetranucleotide repeats (8.2%). Forty primer pairs were designed and tested on 5 strains of P. sojae. Thirty-three primer pairs had successful PCR amplifications. Of the 33 functional primer pairs, 28 primer pairs produced characteristic SSR bands of the expected size, and 15 primer pairs (45.5%) detected polymorphism among 5 tested strains of P. sojae. Based on the polymorphisms detectedwith 20 EST-SSR markers, the 5 tested strains of P. sojae were clustered into 3 groups. In this study, the SSR markers of P. sojae were developed for the first time. These markers could be useful for identification, genetic variation study, and molecularmapping of P. sojae and its relative species.
机译：电子搜索大豆疫霉菌的五千八百个公开可用的表达序列标签（EST），在369个EST中鉴定出415个简单序列重复序列（SSR）。 SSR的平均密度是每8.9 kb筛选的EST序列一个SSR。最频繁的重复是三核苷酸重复（50.1％），最不频繁的是四核苷酸重复（8.2％）。设计了40对引物，并在5个大豆疫霉菌株上进行了测试。 33对引物成功进行了PCR扩增。在33个功能性引物对中，有28个引物对产生了预期大小的特征性SSR条带，在5个测试的大豆疫霉菌株中，有15个引物对（45.5％）检测到多态性。基于检测到的20个EST-SSR标记的多态性，将5个测试过的大豆疫霉菌株分为3组。在这项研究中，首次开发了大豆疫霉菌的SSR标记。这些标记物可用于大豆疫霉及其相关物种的鉴定，遗传变异研究和分子制图。

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