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首页> 外文期刊>Chinese science bulletin >Isolation and characterization of a novel crs-acting sequences regulating root-specific gene from Daucus carota L. LIU Yan, GUO Chang-an, REN
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Isolation and characterization of a novel crs-acting sequences regulating root-specific gene from Daucus carota L. LIU Yan, GUO Chang-an, REN

机译：调控胡萝卜（Daucus carota L）根特异基因的新型crs作用序列的分离与鉴定。刘艳，郭长安，任

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摘要

Aquaporins are ubiquitous channel proteins that facilitate the transport of water across cell membranes. Most of aquaporins are represent in more than one tissues, but some of them performed highest in roots. They are be-lived to participate in water transport. DcRB7, a member of the aquaporin family, was isolated from somatic embryos of carrot and identified as a homologous gene of TobRB7, Further studies showed that the expression of DcRB7 was particular in carrot root. To investigate the transcription regulation of DcRB7, a 650-bp upstream sequence of DcRB7 was isolated by inverse PCR, and was fused to the beta-glucuronidase (GUS) report gene. After the reconibined vectors were transformed into tobacco, the expression pattern was performed by Mstochemical staining and the quantitative analysis of GUS activity. The results indicated that the CK-acting element ofDcRB7 gene directs GUS expression not only as root-specific but also as drought inducible.
机译：水通道蛋白是普遍存在的通道蛋白，可促进水跨细胞膜的运输。水通道蛋白的大多数存在于一个以上的组织中，但其中一些在根部表现最高。他们活着参加水运。从胡萝卜的体细胞胚中分离出水通道蛋白家族成员DcRB7，并将其鉴定为TobRB7的同源基因。进一步的研究表明，DcRB7在胡萝卜根中的表达尤为明显。为了研究DcRB7的转录调控，通过反向PCR分离了650 bp的DcRB7上游序列，并将其与β-葡萄糖醛酸苷酶（GUS）报告基因融合。将重组酶载体转化到烟草中后，通过Mstochemical染色和GUS活性的定量分析进行表达。结果表明，DcRB7基因的CK作用元件不仅指导GUS表达，而且指导其干旱诱导。

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