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首页> 外文期刊>Chinese science bulletin >GCC box in Arabidopsis PDF1.2 promoter is an essential and sufficient cis-acting element in response to MeJA treatment
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GCC box in Arabidopsis PDF1.2 promoter is an essential and sufficient cis-acting element in response to MeJA treatment

机译：拟南芥PDF1.2启动子中的GCC框是响应MeJA处理的必要而充分的顺式作用元件

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摘要

The expression of Arabidopsis PDF1.2 gene is regelated by jasmonic add (JA) and ethylene (ET). It also has been well documented that GCC box is an dement responsive to ET, however, the responsive mechanism of JA in such defense gene expression is unclear. In this paper, the authors define the essential eisaeting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) throughfragment deletions and site-directed mutageneses combining Agrobacterium-medinted transient reporter gene expression in tobacco leaves. Firstly, the MeJA indncible expression of PDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDF 1.2 gene. Secondly, the upstream -300- 243 bp fragment of the promoter was evidenced to respond to MeJA. To future characterize this promoter region, three point mutations were introduced into the -300--243 bp of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like the imperfect palindrome sequence did not significantly decrease MeJA indncible effect, indicating that GCC box in PDF1.2 is for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter expression In response to MeJA. Thus these results indicate that the GCC box in PDF1.2 is an essential and sufficient element to confer MeJA induction.
机译：茉莉添加（JA）和乙烯（ET）使拟南芥PDF1.2基因的表达胶凝。也已经有充分的文献证明GCC盒是对ET作出反应的痴呆，但是，JA在这种防御基因表达中的反应机制尚不清楚。在本文中，作者定义了结合农杆菌介导的瞬时报告基因在烟草叶片中的片段缺失和定点诱变酶对茉莉酸甲酯（MeJA）响应的PDF1.2启动子中必需的eisaeting元素。首先，通过使用PDF 1.2基因的上游-1.86 kb片段确认了PDF 1.2的MeJA不可表达。其次，启动子的上游-300-243 bp片段被证明对MeJA有反应。为了将来表征该启动子区域，在启动子的-300--243 bp中引入了三个点突变。该结果表明，GCC盒的突变消除了MeJA的诱导，而G盒的突变（如不完善的回文序列）并没有显着降低MeJA的不可逆性，表明PDF1.2中的GCC盒是用于MeJA的诱导。通过在CaMV 35S最小启动子上游融合的4xGCC，进一步研究了该GCC盒对MeJA的足够响应性。该结果表明，融合启动子能够响应MeJA而激活报告基因表达。因此，这些结果表明，PDF1.2中的GCC框是授予MeJA感应的必要且充分的元素。

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