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首页> 外文期刊>Chinese science bulletin >Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor
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Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor

机译：高效亲和色谱法和石英晶体微天平生物传感器对肽-肽相互作用的研究

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摘要

The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1-14 of human interferon-beta (hlFN-beta) was chosen as sense peptide and its three antisense peptides (AS-IFN 1, AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08x10~(-4), 1.31x10~(-4) and 2.22x10~(-5) mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction.
机译：通过高效亲和色谱（HPAC）和石英晶体微天平（QCM）生物传感器研究了有义和反义肽之间的特异性相互作用。选择人干扰素-β（hlFN-β）的1-14片段作为有义肽，并根据遗传密码的简并性设计了其三个反义肽（AS-IFN 1，AS-IFN 2和AS-IFN 3）。 。用有义肽作为配体制备亲和柱，并评估亲和色谱行为。 Glu取代的反义肽（AS-IFN 3）在pH 7.5时显示出与固定化有义肽的最强结合。引入了石英微天平-流动注射分析（QCM-FIA）系统来实时研究识别过程。有义肽与AS-IFN 1，AS-IFN 2和AS-IFN 3之间的平衡解离常数分别为2.08x10〜（-4），1.31x10〜（-4）和2.22x10〜（-5）mol / L，分别。机制研究表明，正义肽和AS-IFN 3之间的特异性识别是由于序列依赖性和多峰亲和力相互作用。

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