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Analysis of biomarkers for the cross-linkage of formaldehyde with bovine serum albumin peptides

机译：甲醛与牛血清白蛋白肽交联的生物标志物分析

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Formaldehyde, a well-known environmental toxic hazard, has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine. In diabetes, the activity of SSAO has been found to increase with a subsequent increase in endogenous formaldehyde production. It has been postulated that SSAO-induced production of formaldehyde may be involved in the alteration of protein structure, which may subsequently cause protein deposition associated with chronic pathological disorders. Formaldehyde has also been found to react (cross-link) with amino group of the N-terminal amino acid residue and with the side-chains of arginine, cysteine, histidine and lysine residues. Therefore, formaldehyde may be responsible, at least in part, for protein cross-linkage, oxidative stress and cytotoxicity. The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database. The peptides sequence for control BSA (untreated) digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database. In this way, a total of twenty-seven peptides were matched in the database search query. These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals. Six formaldehyde-treated BSA peptides (FKDLGEEHFK, HLVDEPQNLIK, KVPQVSTPTLVEVSR, RPCFSALTPDETYVPK, LVNELTEFAK, DAFLGSFLYEYSR) were found to be the possible markers for formaldehyde-protein/peptides adducts.
机译：已发现甲醛是一种众所周知的环境毒性危害，它是通过对氨基脲敏感的胺氧化酶催化的甲胺氧化脱氨而内生地产生的。在糖尿病中，已发现SSAO的活性会随着内源甲醛产量的增加而增加。据推测，SSAO诱导的甲醛生产可能与蛋白质结构的改变有关，其随后可能引起与慢性病理疾病相关的蛋白质沉积。还发现甲醛与N-末端氨基酸残基的氨基和精氨酸，半胱氨酸，组氨酸和赖氨酸残基的侧链反应（交联）。因此，甲醛可能至少部分负责蛋白质交联，氧化应激和细胞毒性。使用LC-MS和Mascot数据库研究了甲醛与牛血清白蛋白的交联。通过将MS / MS数据导出到在线MASCOT数据库，在在线数据库搜索查询中匹配了用胰蛋白酶消化的对照BSA（未处理）的肽序列。这样，在数据库搜索查询中总共匹配了27个肽段。然后在所有用不同浓度的FA处理过的胰蛋白酶BSA样品中手动搜索这27个肽段，并在不同的时间间隔内进行孵育。发现六种甲醛处理的BSA肽（FKDLGEEHFK，HLVDEPQNLIK，KVPQVSTPTLVEVSR，RPCFSALTPDETYVPK，LVNELTEFAK，DAFLGSFLYEYSR）是甲醛-蛋白质/肽加合物的可能标记物。

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