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首页> 外文期刊>Chinese science bulletin >Mutagenesis of Ser~(24) of cytochrome b_(559) α subunit affects PSII activities in Chlamydomonas reinhardtii
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Mutagenesis of Ser~(24) of cytochrome b_(559) α subunit affects PSII activities in Chlamydomonas reinhardtii

机译：细胞色素b_（559）α亚基Ser〜（24）的诱变影响莱茵衣藻的PSII活性

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摘要

In order to study the functions of cytochrome b_(559) (Cyt b_(559)) in photosystem two (PSII) activity, mutant S24F of Chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which Ser-ine~(24) (Ser~(24)) locating downstream of Histidine~(23) (His~(23)) in α subunit of Cyt b_(559) was replaced by Phenylalanine (Phe). Physiological and biochemical analysis showed that mutant S24F could be grown photoautotrophically or photoheterotrophically. However, their growth rate was slower either on HSM or TAP medium than that of the control; Analysis of PSII activity revealed that its oxygen evolution was about 71% of wild type (WT); The Photochemical efficiency of PSII (F_v/F_m) of S24F was reduced 0.23-compared with WT; S24F was more sensitive to strong light irradiance than the wild type; Furthermore, SDS-PAGE and Western-blotting analysis indicated that the expression levels of α subunit of Cyt b_(559), LHCII and PsbO of S24F were a little less than those of the wild type. Overall, these data suggests that Ser~(24) plays a significant role in making Cyt b_(559) structure maintain PSII complex activity of oxygen evolution although it is not directly bound to heme group.
机译：为了研究细胞色素b_（559）（Cyt b_（559））在光系统2（PSII）活性中的功能，使用定点诱变构建了莱茵衣藻的S24F突变体，其中Ser-ine〜（24）（Ser Cyt b_（559）α亚基中位于组氨酸〜（23）（His〜（23））下游的〜（24）被苯丙氨酸（Phe）取代。生理生化分析表明，突变体S24F可以光自养或光异养生长。然而，在HSM或TAP培养基上，它们的生长速度都比对照慢。对PSII活性的分析表明，其氧气释放量约为野生型（WT）的71％;与WT相比，S24F的PSII的光化学效率（F_v / F_m）降低了0.23。 S24F对强光辐照比野生型更敏感。此外，SDS-PAGE和Western-blotting分析表明，S24F的Cyt b_（559），LHCII和PsbO的α亚基的表达水平略低于野生型。总体而言，这些数据表明，Ser〜（24）在使Cyt b_（559）结构保持氧释放的PSII复合物活性中起着重要作用，尽管它并不直接与血红素基团结合。

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