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首页> 外文期刊>Chinese science bulletin >Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts
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Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts

机译：野生型和不育两性人体细胞核移植胚泡来源的小鼠胚胎干细胞的比较多能性分析

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摘要

Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi-or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer-ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning.
机译：治疗性克隆，通过体细胞核移植（SCNT）从患者特异性克隆的胚泡中衍生出胚胎干细胞（ESC），对于利用再生医学治疗许多人类疾病具有广阔的前景。畸胎瘤的形成和种系传递已被用于确认小鼠干细胞的多能性，但是由于在伦理上不可能进行种系传递的测试，因此人类胚胎干细胞（hESC）尚未被证明具有充分的多能性。充分多能性的证据。因此，从畸胎瘤内的三个体细胞胚层形成的分化细胞被认为是hESC系中多能性的最佳指标。这些品系缺乏完全的多能性或多能性的可能性尚未得到评估。在这项研究中，我们建立了16个小鼠ESC系，包括3个从不育两性F1小鼠的SCNT囊胚衍生的遗传缺陷核转移ESC（ntESC）系和13个从正常F1小鼠的SCNT囊胚衍生的遗传ESC。我们发现有缺陷的ntESCs在体外表达了所有的多能性标记，并且与正常的ntESCs相比，可能形成畸胎瘤，包括来自所有三个胚层的衍生物，但不能通过生殖系传播。我们的结果表明，尽管使用hESCs的畸胎瘤形成测定在一定程度上定义了多能性，但可能不足以评估其全能性。需要更严格的标准来评估hESC用于治疗性克隆的安全性。

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