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A DNA region of Torulaspora delbrueckii containing the HIS3 gene: sequence, gene order and evolution

机译：含有HIS3基因的Torulaspora delbrueckii的DNA区域：序列，基因顺序和进化

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摘要

We cloned a genomic DNA fragment of the yeast Torulaspora delbrueckii by complementation of a Saccharomyces cerevisiae his3 mutant strain. DNA sequence analysis revealed that the fragment contained two complete ORFs, which share a high similarity with S. cerevisiae His3p and Mrp51p, respectively. The cloned TdHIS3 gene fully complemented the his3 mutation of S. cerevisiae, confirming that it encodes for the imidazoleglycerol-phosphate dehydrate of T. delbrueckii. Two additional ORFs, with a high homology to S. cerevisiae PET56 and DED1 genes, were mapped upstream and downstream from TdHIS3 and TdMRP51, respectively. This genetic organization is analogous to that previously found in Saccharomyces kluyveri and Zygosaccharomyces rouxii. The evolutionary significance of gene order in this chromosomal region is analysed and discussed. The GeneBank Accession No. for the sequenced DNA fragment is AY238990.
机译：我们通过酿酒酵母his3突变菌株的互补克隆了酵母Torulaspora delbrueckii的基因组DNA片段。 DNA序列分析表明该片段包含两个完整的ORF，分别与酿酒酵母His3p和Mrp51p具有高度相似性。克隆的TdHIS3基因与酿酒酵母的his3突变完全互补，从而证实它编码德氏布鲁氏菌的脱水咪唑甘油磷酸酯。与酿酒酵母PET56和DED1基因具有高度同源性的另外两个ORF分别位于TdHIS3和TdMRP51的上游和下游。这种遗传组织类似于先前在克鲁维酵母和鲁氏酵母中发现的遗传组织。分析并讨论了该染色体区域中基因顺序的进化意义。测序的DNA片段的GeneBank登录号为AY238990。

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