Construction and characterization of a series of vectors for Schizosaccharomyces pombe

Adams C; Haldar D; Kamakaka RT

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Construction and characterization of a series of vectors for Schizosaccharomyces pombe

机译：粟酒裂殖酵母一系列载体的构建和表征

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A set of vectors was created to allow cloning and expression studies in Schizosaccharomyces pombe. These vectors had a uniform backbone with an efficient Sz. pombe ARS, ARS3002, but different selectable markers--his3+, leu1+, ade6+ and ura4+. The vectors functioned efficiently as autonomously replicating plasmids that could also be converted into integrating vectors. The ura4+-containing vector was used to construct a Sz. pombe genomic library.

机译：创建了一组载体以允许在粟酒裂殖酵母中进行克隆和表达研究。这些载体具有均匀的骨架和有效的Sz。 pombe ARS，ARS3002，但不同的选择标记-his3 +，leu1 +，ade6 +和ura4 +。载体有效地充当自主复制质粒，也可以将其转化为整合载体。含ura4 +的载体用于构建Sz。 pombe基因组库。

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《Yeast》 |2005年第16期|共8页
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Adams C; Haldar D; Kamakaka RT;
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1. Construction and characterization of a series of vectors for Schizosaccharomyces pombe [J] . Adams C, Haldar D, Kamakaka RT Yeast . 2005,第16期

机译：粟酒裂殖酵母一系列载体的构建和表征
2. Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and New Design of Common Entry Vectors [J] . Yu Zhao, Jef D. Boeke G3: Genes, Genomes, Genetics . 2018,第3期

机译：粟酒裂殖酵母中使用CRISPR-Cas9工具箱的设计者选择标记缺失的构建和通用进入载体的新设计
3. Construction of an expression vector for the fission yeast Schizosaccharomyces pombe [J] . B. Kudla, C. Gaillardin, H. Heslot, Nucleic acids research . 1988,第17期

机译：裂殖酵母裂殖酵母表达载体的构建
4. Signal melding-the construction of training vectors for classifying data series [C] . Lewis, C. . 1995

机译：信号融合-用于分类数据序列的训练向量的构建
5. Genetic construction of malolactic wine yeasts (Schizosaccharomyces pombe, Oenococcus oeni). [D] . Husnik, John. 2002

机译：苹果酸乳酸酵母（Schizosaccharomyces pombe，Oenococcus oeni）的基因构建。
6. Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and New Design of Common Entry Vectors [O] . Yu Zhao, Jef D. Boeke 2018

机译：粟酒裂殖酵母中使用CRISPR-Cas9工具箱的设计者选择标记缺失的构建和通用进入载体的新设计
7. A series of vectors to construct lacZ fusions for the study of gene expression in Schizosaccharomyces pombe [O] . Lafuente Marı́a J, Petit Thomas, Gancedo Carlos 1997

机译：一系列构建lacZ融合蛋白的载体，用于研究粟酒裂殖酵母的基因表达

Construction and characterization of a series of vectors for Schizosaccharomyces pombe

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