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A potential role of the cytoskeleton of Saccharomyces cerevisiae in afunctional organization of glycolytic enzymes

机译：酿酒酵母的细胞骨架在糖酵解酶功能组织中的潜在作用

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Numerous individual enzymes participate in a given synthetic or degradative pathway in which the product of one reaction becomes the substrate for the subsequent enzyme. This raises the question of whether the product of one 'soluble' enzyme diffuses freely through the available cell volume, where it accidentally collides with the subsequent 'soluble' enzyme. Alternatively, enzymes acting in a given pathway may be organized in ordered structures, metabolons. Certain glycolytic enzymes have been shown to co-localize with the cytoskeleton in mammalian cells. We deleted genes coding for proteins associated with the cytoskeleton of Saccharomyces cerevisiae: TPM1 coding for tropomyosin, SAC6 for fimbrin and CIN1 for a microtubule-associated protein. Single deletions or deletions of two such genes had no effect on the specific activities of glycolytic enzymes, or on the rates of glucose consumption and ethanol production. However, the concentrations of glycolytic metabolites during a switch from a gluconeogenic mode of metabolism, growth on an ethanol medium, to glycolysis after glucose addition showed transient deviations from the normal change in metabolite concentrations, as observed in wild type cells. However, all metabolites in mutant strains reached wild-type levels within 2-4 h after the shift. Only ATP levels remained low in all but the tmp1-Delta-sac6-Delta double mutant strains. These observations can be interpreted to mean that metabolic reorganization from a gluconeogenic to a glycolytic metabolism is facilitated by an intact cytoskeleton in yeast. Copyright (C) 1999 John Wiley & Sons, Ltd.
机译：许多单个酶参与给定的合成或降解途径，其中一个反应的产物成为后续酶的底物。这就提出了一个问题，即一种“可溶性”酶的产物是否通过可用的细胞体积自由扩散，从而偶然与随后的“可溶性”酶碰撞。或者，作用于给定途径的酶可以组织成有序的结构，即代谢产物。已经显示某些糖酵解酶与哺乳动物细胞中的细胞骨架共定位。我们删除了编码与酿酒酵母细胞骨架相关的蛋白质的基因：TPM1编码原肌球蛋白，SAC6编码纤维蛋白，CIN1编码微管相关蛋白。单个缺失或两个这样的基因的缺失对糖酵解酶的比活性或葡萄糖消耗和乙醇产生的速率没有影响。然而，如在野生型细胞中所观察到的，在从糖原发生代谢模式，在乙醇培养基上生长到添加葡萄糖后糖酵解的过程中，糖酵解代谢产物的浓度显示出与正常代谢产物浓度变化的瞬时偏差。然而，突变菌株中的所有代谢物在转移后2-4小时内达到野生型水平。除了tmp1-Delta-sac6-Delta双突变株以外，所有ATP中的ATP含量均较低。这些观察结果可以解释为意味着酵母中完整的细胞骨架促进了从糖异生代谢到糖酵解代谢的代谢重组。版权所有（C）1999 John Wiley＆Sons，Ltd.

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