• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Yeast >Allelism of Saccharomyces cerevisiae genes PSO6, involved in survivalafter 3-CPs+UVA, induced damage, and ERG3, encoding the enzyme sterol C-5desaturase
【24h】

Allelism of Saccharomyces cerevisiae genes PSO6, involved in survivalafter 3-CPs+UVA, induced damage, and ERG3, encoding the enzyme sterol C-5desaturase

机译：啤酒酵母基因PSO6的等位基因，参与3-CPs + UVA，诱导的损伤和ERG3的存活，编码固醇C-5去饱和酶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sequencing of the yeast gene that complemented the sensitivity to the photoactivated monofunctional 3-carbethoxypsoralen of the pso6-1 mutant strain revealed that the ERG3 locus, encoding sterol C-5 desaturase involved in biosynthesis of ergosterol, is allelic to PSO6. Disruption of the ERG3 gene yielded an erg3 Delta mutant viable in ergosterol-containing YEPD media with the same pleiotropic mutant phenotype known for pso6-1 and erg3 mutants, including sensitivity to hydrogen peroxide and paraquat. Thus, the erg3/pso6 yeast mutant seems to be more sensitive than the WT to 3-CPs + UVA because of the oxidative damage contributed by this treatment and not because of an impaired repair of the furocoumarin-thymine monoadducts formed in the DNA. We found a significant increase of petites amongst erg3 Delta and pso6-1 yeast mutant strains grown in conditions where respiration was mandatory. Mutant pso6-1, with its lowered content of ergosterol, exhibited enhanced synthesis of chitin that was maldistributed and not confined to the bud scars. Chitin overproduction in pso6/erg3 mutants resulted in hypersensitivity to Calcofluor White. Copyright (C) 1999 John Wiley & Sons, Ltd.
机译：补充对pso6-1突变菌株的光活化单官能3-碳乙氧基补骨脂素的敏感性的酵母基因测序表明，编码参与麦角固醇生物合成的固醇C-5去饱和酶的ERG3基因座与PSO6等位基因。 ERG3基因的破坏产生了一个erg3 Delta突变体，该突变体在含麦角固醇的YEPD培养基中具有与pso6-1和erg3突变体相同的多效突变体表型，包括对过氧化氢和百草枯的敏感性。因此，erg3 / pso6酵母突变体似乎比WT对3-CPs + UVA更敏感，这是由于这种处理所造成的氧化损伤，而不是因为DNA中形成的呋喃香豆素-胸腺嘧啶单加合物的修复受损。我们发现在强制呼吸的条件下生长的erg3 Delta和pso6-1酵母突变株之间的小身形显着增加。突变体pso6-1的麦角固醇含量降低，显示出几丁质的合成增强，几丁质的分布不均且不局限于芽疤。 pso6 / erg3突变体中几丁质的过量生产导致对Calcofluor White过敏。版权所有（C）1999 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号