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首页> 外文期刊>Yeast >Mutations in the Lcb2p subunit of serine palmitoyltransferase eliminate the requirement for the TSC3 gene in Saccharomyces cerevisiae
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Mutations in the Lcb2p subunit of serine palmitoyltransferase eliminate the requirement for the TSC3 gene in Saccharomyces cerevisiae

机译：丝氨酸棕榈酰转移酶Lcb2p亚基的突变消除了酿酒酵母中TSC3基因的需要

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摘要

Serine palmitoyltransferase catalyses the committed step in sphingolipid synthesis, the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine. Two proteins, Lcb1p and Lcb2p, are essential for enzyme activity and a third protein, the 80-amino acid Tsc3p, stimulates the activity of serine palmitoyltransferase several-fold. Tsc3p physically associates with a complex of Lcb1p-Lcb2p and stimulates enzyme activity posttranslationally, but its precise function is not known. Tsc3p is essential for cell viability only at elevated temperatures, although serine palmitoyltransferase activity is reduced in the tsc3Delta mutant, even at permissive growth temperatures. Tsc3p is apparently not required for any essential process besides stimulation of serine palmitoyltransferase at 37degreesC, since providing sphingoid bases to the growth medium reverses the temperature-sensitive growth phenotype of the tsc3Delta mutant. To gain further insight into the function of Tsc3p, suppressor mutants that eliminate the Tsc3p requirement for growth at 37degreesC were isolated and characterized. These studies show that dominant mutations in the Lcb2p subunit of serine palmitoyltransferase suppress the temperature-sensitive growth phenotype of the tsc3Delta null mutant by increasing the Tsc3p-independent serine palmitoyltransferase activity. Copyright
机译：丝氨酸棕榈酰转移酶催化鞘脂合成中的重要步骤，即丝氨酸与棕榈酰辅酶A缩合形成3-酮基鞘氨醇。 Lcb1p和Lcb2p这两种蛋白质是酶活性必不可少的，而另一种蛋白质是80个氨基酸的Tsc3p，可以使丝氨酸棕榈酰转移酶的活性提高几倍。 Tsc3p在物理上与Lcb1p-Lcb2p的复合物缔合，并在翻译后刺激酶的活性，但其确切功能尚不清楚。尽管即使在允许的生长温度下，tsc3Delta突变体中丝氨酸棕榈酰转移酶的活性也会降低，但Tsc3p仅在高温下才对细胞活力至关重要。除了在37℃刺激丝氨酸棕榈酰转移酶外，任何必要的过程显然都不需要Tsc3p，因为向生长培养基提供鞘氨醇碱基可逆转tsc3Delta突变体对温度敏感的生长表型。为了进一步了解Tsc3p的功能，分离并鉴定了消除Tsc3p在37℃下生长所需的抑制突变体。这些研究表明，丝氨酸棕榈酰转移酶的Lcb2p亚基中的显性突变通过增加独立于Tsc3p的丝氨酸棕榈酰转移酶的活性，抑制了tsc3Delta null突变体的温度敏感性生长表型。版权

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