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首页> 外文期刊>Chemistry and Physics of Lipids >Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures
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Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures

机译：黄酮类化合物对二元脂质混合物形成的巨大单层囊泡相分离的影响

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摘要

Confocal fluorescence microscopy have been employed to investigate phase separation in giant unilamellar vesicles prepared from binary mixtures of unsaturated dioleoylphosphocholine with saturated phosphocholines or brain sphingomyelin in the absence and presence of the flavonoids, biochanin A, phloretin, and myricetin. It has been demonstrated that biochanin A and phloretin make uncolored domains more circular or eliminate visible phase separation in liposomes while myricetin remains the irregular shape of fluorescence probe-excluding domains. Influence of the flavonoids on the endotherms of liposome suspension composed of dioleoylphosphocholine and dimyristoylphosphocholine was investigated by the differential scanning calorimetry. Calorimetry data do not contradict to confocal imaging results.
机译：共焦荧光显微镜已用于研究在不存在和存在类黄酮，生物增生素A，促绿素和杨梅素的情况下，由不饱和油酰磷脂酰胆碱与饱和磷酸胆碱或脑鞘磷脂的二元混合物制得的巨大单层囊泡中的相分离。已经证明，生物chanin A和荧光素使脂质体中的无色域更圆或消除了可见的相分离，而杨梅素则保留了荧光探针除外域的不规则形状。通过差示扫描量热法研究了类黄酮对由二油酰基磷酸胆碱和二肉豆蔻酰基磷酸胆碱组成的脂质体悬液的吸热的影响。量热数据与共焦成像结果并不矛盾。

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