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首页> 外文期刊>Hepatology: Official Journal of the American Association for the Study of Liver Diseases >Bioluminescence imaging allows measuring CD8 T cell function in the liver.
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Bioluminescence imaging allows measuring CD8 T cell function in the liver.

机译：生物发光成像可以测量肝脏中的CD8 T细胞功能。

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摘要

In vivo evaluation of CD8 T cell effector (cytotoxic T lymphocyte [CTL]) function in peripheral organs such as the liver is currently not possible but would greatly improve our understanding of local immune regulation, because simple determination of antigen-specific CTL numbers does not predict the outcome of immune responses. In particular, measurement of alanine aminotransferase serum levels is not sensitive enough to detect T cell immunity against low numbers of target hepatocytes. We developed a procedure that detects virus-specific effector function of CTLs in the liver after simultaneous adenoviral transfer of reporter and immune target genes into hepatocytes, followed by bioluminescence imaging of reporter genes. Bioluminescence imaging enabled detection of as few as 10,000 infected hepatocytes in vivo, and even more importantly, quantification of antiviral effector function of as few as 50,000 CTLs. Conclusion: Our results provide evidence that low numbers of antigen-specific CTLs are sufficient to control viral gene expression and eliminate viral infection from hepatocytes. The experimental system established here is a highly sensitive method to simultaneously detect viral infection of hepatocytes and to quantify antiviral CTL function in the liver in vivo and will help in characterizing principles of hepatic immune regulation.
机译：目前尚不可能在体内评估外周器官（例如肝脏）中的CD8 T细胞效应子（细胞毒性T淋巴细胞[CTL]）功能，但会大大提高我们对局部免疫调节的了解，因为不能简单地确定抗原特异性CTL数量预测免疫反应的结果。尤其是，丙氨酸转氨酶血清水平的测量不够灵敏，无法检测出针对少量靶肝细胞的T细胞免疫。我们开发了一种程序，用于在腺病毒同时将报告基因和免疫靶基因同时转移到肝细胞后，检测报告基因的生物发光成像，从而检测肝脏中CTL的病毒特异性效应子功能。生物发光成像能够在体内检测到多达10,000个受感染的肝细胞，甚至更重要的是，定量化的抗病毒效应子功能可以多达50,000个CTL。结论：我们的结果提供了证据，即少量的抗原特异性CTL足以控制病毒基因表达并消除肝细胞中的病毒感染。此处建立的实验系统是一种高度灵敏的方法，可以同时检测肝细胞的病毒感染并在体内量化肝脏中抗病毒CTL的功能，并将有助于表征肝免疫调节的原理。

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