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首页> 外文期刊>Hepatology: Official Journal of the American Association for the Study of Liver Diseases >Genotype 4 hepatitis C virus: beware of false-negative RNA detection.
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Genotype 4 hepatitis C virus: beware of false-negative RNA detection.

机译：基因型4丙型肝炎病毒：提防假阴性RNA检测。

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摘要

We have followed with interest the debate regarding the ability of the COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) hepatitis C virus (HCV) test (Roche, Meylan, France) to accurately detect and quantify genotype 4 HCV, We recently identified seven genotype 4 samples [4h (4); 4k (2); 41 (1)] from HCV antibody-positive patients; we repeatedly found them HCV RNA undetectable with CAP/CTM, but we discovered viral loads greater than 5 log_(10) IU/mL with the Abbott RealTime HCV assay (Abbott, Rungis, France). When the 5'-non-coding gene of these undetected samples was compared to sequences from 29 genotype 4 samples [4 (5); 4a (6); 4c (1); 4d (9); 4f (1); 4g (2); 4h (2); 4k (2); 4r (1)], significant sequence differences between underquantified samples (difference between the two assays > 1 logio IU/mL), undetected strains, and samples with comparable viral loads were identified at positions 145 (P < 0.0001), 165 (P < 0.0001), 203 (P < 0.0001), and 204 (P = 0.0002) with the chi-square test. Positions 203 and 204 represent a nucleotide insertion in a few subtypes (f, g, h, k, o, p, and q) and are unlikely to play a role in CAP/CTM underquantification. However, any mutation at position 145 (rarely described) could dramatically impair the performance of the Roche assay, whereas a mutated nucleotide at position 165 (constantly found in subtypes g, h, k, 1, m, o, and q) leads to decreased quantification of many genotype 4 subtypes.
机译：我们感兴趣地关注了关于COBAS AmpliPrep / COBAS TaqMan（CAP / CTM）丙型肝炎病毒（HCV）测试（Roche，Meylan，法国）准确检测和定量4型HCV的能力的争论，我们最近确定了7个基因型4个样本[4h（4）; 4k（2）; 41（1）]来自HCV抗体阳性患者;我们反复发现它们是用CAP / CTM无法检测到的HCV RNA，但是我们发现使用Abbott RealTime HCV检测法（法国，Abbott，Abbott）的病毒载量大于5 log_（10）IU / mL。当将这些未检测到的样品的5'-非编码基因与29个基因型4样品的序列进行比较时[4（5）; 4a（6）; 4c（1）; 4d（9）; 4f（1）; 4克（2）; 4h（2）; 4k（2）; [4r（1）]，在位置145（P <0.0001），165（P <0.001）处鉴定出未量化样品之间的显着序列差异（两次测定之间的差异> 1 logio IU / mL），未检测到的菌株以及具有可比病毒载量的样品。卡方检验，结果为0.0001），203（P <0.0001）和204（P = 0.0002）。位置203和204代表一些亚型（f，g，h，k，o，p和q）中的核苷酸插入，并且不太可能在CAP / CTM定量不足中起作用。但是，第145位的突变（很少描述）会极大地损害Roche分析的性能，而第165位的突变核苷酸（在g，h，k，1，m，o和q亚型中经常发现）会导致降低了许多基因型4亚型的定量。

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