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首页> 外文期刊>Heredity: An International Journal of Genetics >Molecular characterisation of sweet cherry (Prunus avium L.) genotypes using peach [Prunus persica (L.) Batsch] SSR sequences
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Molecular characterisation of sweet cherry (Prunus avium L.) genotypes using peach [Prunus persica (L.) Batsch] SSR sequences

机译：利用桃[Prunus persica（L.）Batsch] SSR序列对甜樱桃（Prunus avium L.）基因型进行分子表征

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A total of 76 sweet cherry genotypes were screened with 34 microsatellite primer pairs previously developed in peach. Amplification of SSR loci was obtained for 24 of the microsatellite primer pairs, and 14 of them produced polymorphic amplification patterns. On the basis of polymorphism and quality of amplification, a set of nine primer pairs and the resulting 27 informative alleles were used to identify 72 genotype profiles. Of these, 68 correspond to unique cultivar genotypes, and the remaining four correspond to three cultivars that could not be differentiated from the two original genotypes of which they are mutants, and two very closely related cultivars. The mean number of alleles per locus was 3.7 while the mean heterozygosity over the nine polymorphic loci averaged 0.49. The results demonstrate the usefulness of cross-species transferability of microsatellite sequences allowing the discrimination of different genotypes of a fruit tree species with sequences developed in other species of the same genus. UPGMA cluster analysis of the similarity data divided the ancient genotypes studied into two fairly well-defined groups that reflect their geographic origin, one with genotypes originating in southern Europe and the other with the genotypes from northern Europe and North America. [References: 44]
机译：用34种先前在桃子中发育的微卫星引物对筛选了76种甜樱桃基因型。对24个微卫星引物对获得了SSR基因座的扩增，其中14对产生了多态性扩增模式。基于多态性和扩增质量，使用一组九对引物和由此产生的27个信息性等位基因来鉴定72个基因型谱。其中68个对应于独特的品种基因型，其余四个对应于无法与它们是突变体的两个原始基因型和两个密切相关的品种区分开的三个品种。每个基因座的等位基因平均数为3.7，而9个多态位点的平均杂合度为0.49。结果证明了微卫星序列的跨物种转移性的有用性，其允许区分具有相同属的其他物种中发展的序列的果树物种的不同基因型。 UPGMA对相似性数据的聚类分析将所研究的古老基因型分为两个相当明确的组，以反映其地理起源，一个具有源自欧洲南部的基因型，另一个具有来自北欧和北美的基因型。 [参考：44]

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