Monoclonal antibody against mouse CAR following genetic immunization.

Carson SD; Switzer BL; Tracy SM; Chapman NM

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Monoclonal antibody against mouse CAR following genetic immunization.

机译：基因免疫后针对小鼠CAR的单克隆抗体。

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To broaden our repertoire of monoclonal antibodies against CAR (coxsackievirus and adenovirus receptor), we inoculated mice with an expression vector containing the cDNA encoding human CAR extracellular and transmembrane sequence, and boosted the response by inoculation with soluble human CAR protein produced in E. coli. Of the hybridomas obtained following this immunization protocol, one secreted IgG with exceptional reactivity against mouse CAR. Since CAR has been shown to form dimers, expression of human CAR in cells that express mouse CAR may have stimulated the host immune system to recognize endogenous CAR in heterodimers.

机译：为了扩大我们的抗CAR（柯萨奇病毒和腺病毒受体）单克隆抗体库，我们用包含编码人CAR细胞外和跨膜序列cDNA的表达载体接种了小鼠，并通过接种大肠杆菌中产生的可溶性人CAR蛋白来增强了反应。。通过该免疫方案获得的杂交瘤中，有一个分泌的IgG对小鼠CAR具有极强的反应活性。由于已显示CAR形成二聚体，因此在表达小鼠CAR的细胞中人CAR的表达可能刺激了宿主免疫系统识别异源二聚体中的内源CAR。

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《Hybridoma and hybridomics》 |2004年第1期|共4页
作者
Carson SD; Switzer BL; Tracy SM; Chapman NM;
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正文语种 eng
中图分类肿瘤学;
关键词
Laboratory mice; Immunization; Human; Antibodies; Monoclonal; Genetic; 免疫法; 抗体; 单克隆;

机译：Laboratory mice;Immunization;Human;Antibodies;Monoclonal;Genetic;免疫法;抗体;单克隆;

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1. Monoclonal antibody against mouse CAR following genetic immunization. [J] . Carson SD, Switzer BL, Tracy SM, Hybridoma and hybridomics . 2004,第1期

机译：基因免疫后针对小鼠CAR的单克隆抗体。
2. Generation of a new monoclonal antibody against MALT1 by genetic immunization. [J] . Maestre L, Fontan L, Martinez-Climent JA, Hybridoma . 2007,第2期

机译：通过基因免疫产生针对MALT1的新型单克隆抗体。
3. Monoclonal anti-thrombopoietin antibodies generated by genetic immunization. [J] . Lim NK, Kim JH, Kim SY, Hybridoma . 2006,第2期

机译：通过基因免疫产生的单克隆抗血小板生成素抗体。
4. Developing SISCAPA-MRM-MS biomarker verification technology including a comparison between rabbit polyclonal, mouse monoclonal, and rabbit monoclonal antibodies [C] . Regine M. Schoenherr, Li-chia Feng, Lei Zhao, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：制定SISCAPA-MRM-MS Biomarker验证技术，包括兔多克隆，小鼠单克隆和兔单克隆抗体之间的比较
5. Humanizing the jaa-f11 mouse monoclonal antibody. [D] . Eng, Jing Ying. 2014

机译：人源化jaa-f11小鼠单克隆抗体。
6. A common anti-cardiolipin antibody idiotype in autoimmune disease: identification using a mouse monoclonal antibody directed against a naturally-occurring anti-phospholipid antibody. [O] . M Sutjita, A Hohmann, R Comacchio, 1989

机译：自身免疫性疾病中常见的抗心磷脂抗体独特型：使用针对天然存在的抗磷脂抗体的小鼠单克隆抗体进行鉴定。
7. A New Monoclonal Antibody that Recognizes 180 kDa Polypeptide Expressed on Early Mouse Embryos and Mouse Embryonal Carcinoma Cells. (embryonal carcinoma/monoclonal antibody/intrasplenic primary immunization/180 kDa polypeptide) [O] . Kenji Abe, Nobuo Takagi, Masaharu Naiki, 1990

机译：一种新的单克隆抗体，其识别在早期小鼠胚胎和小鼠胚胎癌细胞上表达的180kDa多肽。（胚胎癌/单克隆抗体/血糖丙烯原发性免疫/ 180kDa多肽）
8. Mouse Monoclonal Antibody Specific for an Allotypic Determinant of the Igh(a) Allele of Murine IgM: Genetic and Functional Analysis of Igh-6a Epitopes Using Anti-IgM Monoclonal Antibodies. [R] . Sieckmann, D. G., Stall, A. M., Subbarao, B. 1991

机译：小鼠单克隆抗体特异性针对Igh（a）小鼠Igm等位基因的同种型决定因素：使用抗Igm单克隆抗体的Igh-6a表位的遗传和功能分析。

Monoclonal antibody against mouse CAR following genetic immunization.

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