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首页> 外文期刊>HortScience >Determining the Optimal Timing of Fruit Harvest in Transgenic Tomato Expressing F1-V, a Candidate Subunit Vaccine against Plague.
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Determining the Optimal Timing of Fruit Harvest in Transgenic Tomato Expressing F1-V, a Candidate Subunit Vaccine against Plague.

机译：确定表达F1-V（抗瘟疫的候选亚单位疫苗）的转基因番茄中水果收获的最佳时机。

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摘要

Changes in the amounts of F1-V, an antigen fusion protein and a candidate subunit vaccine against plague, and total soluble protein (TSP) in green fruit of transgenic tomato plants were investigated to identify the optimum harvest timing to maximize the F1-V yields. Two T2 progenies of the transgenic plant, 22.11.21 and 22.11.5, were grown. The F1-V concentration rapidly decreased at the beginning of the green stage and decreased to less than 5% of the initial concentration at the late green stage in 22.11.21. The F1-V concentration also decreased as fruit size increased in 22.11.5, but the pattern of the decrease was linear and different from that in 22.11.21. The concentration of TSP also decreased with fruit growing in both plants. When calculated on a whole fruit basis, the F1-V content linearly decreased with increasing fruit size in 22.11.21. In 22.11.5, the F1-V content per fruit also tended to decrease from the middle to late green stage. Based on these observations, collecting small green fruits without pruning was proposed as a harvest practice that may maximize the F1-V yields. Thus, the optimum protocols for harvesting and pruning for plant-made pharmaceutical production may be substantially different from those currently used in commercial hydroponic greenhouses for fresh market tomato.
机译：研究了转基因番茄植株绿色水果中F1-V，抗原融合蛋白和抗鼠疫候选亚单位疫苗以及总可溶性蛋白（TSP）含量的变化，以确定最佳收获时机，以最大化F1-V产量。种植了转基因植物的两个T2后代22.11.21和22.11.5。 F1-V浓度在绿色阶段开始时迅速下降，并在22.11.21的绿色后期阶段下降到小于初始浓度的5％。随着果实大小的增加，F1-V的浓度也在22.11.5中降低，但下降的趋势是线性的，并且与22.11.21中的下降趋势不同。随着两种植物果实的生长，TSP的浓度也降低。当以整个水果为基础计算时，F1-V含量随水果大小的增加而线性降低（22.11.21）。在22.11.5中，每个水果的F1-V含量也从中绿期到后期绿色阶段都有下降的趋势。基于这些观察结果，提出了不修剪即可收集小的绿色水果的收成方法，以使F1-V的产量最大化。因此，用于植物药物生产的收获和修剪的最佳方案可能与目前用于新鲜市场番茄的商业水培温室中使用的方案大不相同。

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