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Seed germination of huagaimu, a critically endangered plant endemic to southeastern Yunnan, China.

机译:huagaimu的种子发芽,huagaimu是中国云南东南部特有的极度濒危植物。

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As a critically endangered tree in the Magnoliaceae family, huagaimu (Manglietiastrum sinicum) is represented by only 10 mature individuals in evergreen broadleaved montane forests of southeastern Yunnan Province, China. Our previous work revealed the existence of a seed dormancy period for this species. The current study was performed to evaluate the effects of plant growth regulators (PGRs) and moist chilling on breaking seed dormancy in this species. Germination of seeds pretreated for 24 h with gibberellic acid (GA3), alpha -naphthaleneacetic acid, 6-benzyladenine, and 2,4-dichlorophenoxyacetic acid indicated that only GA3, at concentrations of 300 and 500 mg.L-1, can significantly break the seed dormancy of huagaimu after 50 days of incubation, with about 66% germination under 500 mg.L-1 GA3. Moist chilling at 4 degrees C for 3 weeks can also effectively break the seed dormancy of the species, with 56% of seeds treated in this way germinating after 30 days of incubation. The combined treatments of PGRs followed by moist chilling were also conducted. Based on germination results after 30 days of incubation, the seed germination of combined treatments was significantly higher than that of PGR treatments. However, the seeds treated only with moist chilling presented the highest germination percentage among all the treatments.
机译:作为木兰科中一种极度濒危的树,在中国云南东南部常绿阔叶山地森林中,花盖木(Manglietiastrum sinicum )仅由10个成熟个体代表。我们以前的工作表明该物种存在种子休眠期。进行当前的研究以评估植物生长调节剂(PGR)和湿冷对打破该物种种子休眠的影响。用赤霉素(GA 3 ),α-萘乙酸,6-苄基腺嘌呤和2,4-二氯苯氧基乙酸预处理24 h的种子发芽表明只有GA 3 分别在300和500 mg.L -1 的浓度下孵育50天后,可以显着打破花改木的种子休眠,在500 mg.L -1 < / sup> GA 3 。在4摄氏度下湿冷3周也可以有效打破该物种的种子休眠状态,以这种方式处理的种子中有56%在孵育30天后萌发。还进行了PGR的组合处理,然后进行湿冷。根据孵育30天后的发芽结果,联合处理的种子发芽率显着高于PGR处理。然而,在所有处理中,仅用湿冷处理的种子发芽率最高。

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