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Multiwalled Carbon Nanotubes for Amperometric Array-Based Biosensors

机译：用于基于安培阵列的生物传感器的多壁碳纳米管。

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摘要

For diagnostic and therapeutic purposes an accurate determination of multiple metabolites is often required. Amperometric devices are attractive tools to quantify biological compounds due to the direct conversion of a biochemical event to a current. This review addresses recent developments in the use of multiwalled carbon nanotubes to enhance detection capability of amperometric array-based biosensors. More specifically, the principal techniques for multiwalled carbon nanotube incorporation onto microelectrode arrays are described. In these types of devices, each electrode is responsible for sensing one metabolite. The specificity is often given by an enzyme since most biomolecules are not electroactive compounds. Common strategies for the protein immobilization onto multiwalled carbon nanotubes are also presented. After the discussion of nanotube/biomolecule integration onto electrode surfaces, three results are shown. The first one regards the influence on the biodetection signal of differently oriented multiwalled carbon nanotubes. Secondly, a demonstration of enhanced biodetection parameters by using multiwalled carbon nanotubes is given. Finally, a comparative study of three enzymes used to detect the same metabolite and adsorbed onto multiwalled carbon nanotubes is also reported.
机译：为了诊断和治疗目的，经常需要准确测定多种代谢物。由于生化事件直接转换为电流，安培仪器是用于量化生物化合物的有吸引力的工具。这篇评论解决了使用多壁碳纳米管增强基于安培阵列的生物传感器的检测能力的最新进展。更具体地，描述了用于将多壁碳纳米管结合到微电极阵列上的主要技术。在这些类型的设备中，每个电极负责感测一种代谢物。由于大多数生物分子不是电活性化合物，因此通常由酶来提供特异性。还介绍了将蛋白质固定在多壁碳纳米管上的常见策略。在讨论了将纳米管/生物分子整合到电极表面后，显示了三个结果。第一个是关于取向不同的多壁碳纳米管对生物检测信号的影响。其次，给出了使用多壁碳纳米管增强生物检测参数的演示。最后，还报道了三种酶的比较研究，这些酶用于检测相同的代谢物并吸附在多壁碳纳米管上。

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