• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Human Reproduction >A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers.
【24h】

A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers.

机译：一项前瞻性随机研究，用于评估冻融胚胎移植前部分透明带消化的益处。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Assisted hatching (AH) in fresh embryo transfer (ET) could be associated with increased implantation rates. However, very few prospective randomized studies have specifically addressed the issue of AH during frozen-thawed embryo transfers (FET) cycles, those that have reported controversial results. The aim of this study was to evaluate the benefit of an enzymatic zona pellucida treatment of frozen-thawed embryos before transfer. METHODS: This was a prospective study including 125 non-donor FET cycles from 125 infertile couples. FETs were randomly allocated into AH group (n = 61, embryos pretreated with pronase 5 IU/ml for 1 min at 37 degrees C) or control group (n = 64, untreated embryos). Zona pellucida thickness was measured for each transferred embryo. The main outcome parameters were clinical pregnancy and implantation rates. RESULTS: The two groups were comparable regarding mean women's age, duration and indications of infertility, IVF outcome after fresh ETs, numbers and quality of fresh and frozen embryos, frozen-thawed embryo survival rates and blastomeres survival indexes. Despite a statistically significant decrease of zona pellucida thickness after pronase treatment [(mean +/- SD) 18.5 +/- 2.25 versus 14.5 +/- 2.75 microm; P < 0.0001], implantation (9.6 versus 9.2%) and clinical pregnancy rates (18.0 versus 17.2%) were not statistically different after FETs, with a similar mean number of embryos transferred between AH and control groups, respectively. CONCLUSION: Within the constraints of our protocol, partial enzymatic digestion of zona pellucida by pronase was not related with any benefit of the FET outcome especially concerning the implantation ability of frozen-thawed embryos.
机译：背景：新鲜胚胎移植（ET）中的辅助孵化（AH）可能与植入率增加有关。但是，很少有前瞻性随机研究专门研究过冷冻解冻胚胎移植（FET）周期中的AH问题，这些研究报告了有争议的结果。这项研究的目的是评估转移前酶解透明带处理冻融胚胎的益处。方法：这是一项前瞻性研究，包括来自125个不育夫妇的125个非供体FET周期。将FETs随机分为AH组（n = 61，在37摄氏度下用5 IU / ml的pronase预处理1分钟）或对照组（n = 64，未处理的胚胎）。测量每个转移胚胎的透明带厚度。主要结局参数是临床妊娠率和着床率。结果：两组在平均女性年龄，不孕持续时间和适应症，新鲜ET后的IVF结果，新鲜和冷冻胚胎的数量和质量，冷冻融化的胚胎存活率和卵裂球存活指数方面具有可比性。尽管pronase处理后透明带厚度有统计学上的显着降低[（平均+/- SD）18.5 +/- 2.25微米对14.5 +/- 2.75微米; P <0.0001]，FET后的植入率（9.6％vs. 9.2％）和临床妊娠率（18.0％vs 17.2％）在统计学上没有差异，分别在AH组和对照组之间有相似的平均胚胎数。结论：在我们的方案的约束下，链酶对部分透明带的酶促消化与FET结局没有任何好处，特别是关于冷冻融化胚胎的植入能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号