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首页> 外文期刊>Human and Experimental Toxicology >Is there a role for PCR-SSCP among the methods for missense mutation detection of TP53 gene?
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Is there a role for PCR-SSCP among the methods for missense mutation detection of TP53 gene?

机译：在TP53基因错义突变检测方法中，PCR-SSCP是否起作用？

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摘要

Mutation analysis methods have increased in variety during the past years. High-throughput microarray methods have especially increased in popularity. However, new methods require reference points, and not all of the methods are equal in sensitivity and specificity. Furthermore, the detection of unknown missense mutations, such as unknown TP53 mutations in human tumors, for clinical purposes requires great accuracy, which may be difficult to acquire with the current high-throughput methods. For these reasons, the classical methods, such as PCR-manual sequencing and PCR-SSCP, are still valuable and necessary.
机译：在过去几年中，变异分析方法的种类有所增加。高通量微阵列方法尤其流行。但是，新方法需要参考点，并且并非所有方法的敏感性和特异性都相同。此外，出于临床目的，未知的错义突变（例如人肿瘤中的未知TP53突变）的检测需要很高的准确性，而使用当前的高通量方法可能很难获得这种准确性。由于这些原因，传统的方法（例如PCR手动测序和PCR-SSCP）仍然是有价值且必要的。

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