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首页> 外文期刊>Zoonoses and Public Health >Predominance and persistence of a single clone of Listeria ivanovii in a Manchego cheese factory over 6 months.
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Predominance and persistence of a single clone of Listeria ivanovii in a Manchego cheese factory over 6 months.

机译：在Manchego奶酪工厂中，超过六个月的伊夫氏李斯特菌单个克隆的优势和持久性。

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摘要

In an attempt to study the diversity and persistence of molecular subtypes of pathogenic Listeria spp. in a cheese factory at the La Mancha region of Spain, 43 samples were taken from incoming raw milk (cow's, ewe's, goat's and mixed species) and from certain food-contact and environmental surfaces before and/or after sanitation. Of these samples, 12 contained pathogenic Listeria. From the chromogenic agar plates corresponding to those, 46 phosphatidylinositol-specific phospholipase C-positive isolates were randomly taken for further analysis, including biochemical tests and pulsed-field gel electrophoresis (PFGE). They coincided in identifying all the 46 as Listeria ivanovii subsp. ivanovii, apparently a single PFGE type. Both ewe's and goat's raw milk batches from asymptomatic animals tested along the 6-month period persistently carried the same strain, which was also obtained from inner surfaces of raw milk truck tanks and the milk dump tank at the cheese factory. Biofilm-forming abilities of this L. ivanovii clone and interference against L. monocytogenes Scott A reference strain were tested, but failed to account for the clone's apparent pervasive presence
机译：为了研究致病性李斯特菌属的分子亚型的多样性和持久性。在西班牙拉曼恰地区的一家奶酪工厂中，从卫生设施之前和/或之后从进来的原奶（牛，母羊，山羊奶和混合品种）以及某些与食物接触和环境的表面中抽取了43个样品。在这些样品中，有12个包含致病性李斯特菌。从相应的发色琼脂平板中，随机抽取46种磷脂酰肌醇特异性磷脂酶C阳性分离物进行进一步分析，包括生化测试和脉冲场凝胶电泳（PFGE）。他们碰巧将所有46种都鉴定为伊斯特氏菌亚种。 ivanovii，显然是单个PFGE类型。在六个月的时间内测试的无症状动物的母羊和山羊原奶批次均持续携带相同的菌株，这也是从奶酪工厂的原奶卡车罐和卸奶罐的内表面获得的。测试了该伊凡诺氏乳杆菌克隆的生物膜形成能力和对单核细胞增生李斯特菌Scott A参考菌株的干扰，但未能解释该克隆明显存在的原因

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