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首页> 外文期刊>Zoological Science >Developmental stage-dependent expression of troponin T isoforms in chickenembryonic breast muscles grafted on chorio-allantoic membrane
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Developmental stage-dependent expression of troponin T isoforms in chickenembryonic breast muscles grafted on chorio-allantoic membrane

机译：绒毛膜-尿囊膜移植鸡胚乳腺肌钙蛋白T同工型的发育阶段依赖性表达

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摘要

To examine whether the expression pattern of muscle-specific protein in embryonic muscle tissues at the different developmental stages depends on the types of myogenic cells, chicken breast muscle (pectoralis major) tissues of 10-day, 14-day, and 18-day old embryos (E10, E14, and E18, respectively) were grafted on chorio-allantoic membrane (CAM) of 9-day-old chicken embryos and cultured for 12 days at the longest, since the chorio-allantoic grafting is useful in pursuing muscle-cell lineage during muscle differentiation. The muscle fiber formation and expression of troponin T (TnT) isoforms in grafts were investigated by histological and immunohistochemical methods with anti-fast-muscle-type and anti-slow-muscle-type TnT (rabbit sera), In grafts of E10 breast muscle, most muscle fibers continued to develop without degeneration and TnT isoform expression of the fibers changed from the concomitant expression of fast-muscle-type (F-type) and slow-muscle-type (S-type) to the expression of F-type only. In grafts of E14 and E18 breast muscles, the muscle fibers first degenerated with pyknotic nuclei and hyaline cytoplasm, and then new muscle fibers expressing F-type TnT isoforms were formed by the fusion of basophilic cells. The new muscle fibers in grafts of E14 muscles were different from those of E18 ones in that the former also expressed S-type TnT isoforms. In this paper, developmental stage-dependent TnT isoform expression of the embryonic breast muscles grafted on CAM is discussed in connection with cell-type difference of grafted muscle cells.
机译：要检查不同发育阶段胚胎肌肉组织中肌肉特异性蛋白的表达模式是否取决于成肌细胞的类型，10日龄，14日龄和18日龄的鸡胸肌（胸大肌）组织将胚胎（分别为E10，E14和E18）移植到9天大的鸡胚的绒毛-尿囊膜（CAM）上，并最长培养12天，因为绒毛-尿囊的移植可用于追求肌肉肌肉分化过程中的细胞谱系。通过组织学和免疫组织化学方法，用抗快肌型和抗慢肌型TnT（兔血清），对E10乳腺移植物中的肌纤维形成和肌钙蛋白T（TnT）同工型表达进行了研究。 ，大多数肌肉纤维继续发育而不会变性，并且纤维的TnT同工型表达从快肌型（F型）和慢肌型（S型）的同时表达变为F型只要。在E14和E18胸肌的移植物中，肌肉纤维首先被致死性核和透明质细胞质变性，然后通过嗜碱细胞的融合形成表达F型TnT同工型的新肌肉纤维。 E14肌肉移植物中的新肌肉纤维与E18肌肉中的新纤维不同，前者也表达S型TnT亚型。在本文中，结合移植的肌肉细胞的细胞类型差异，讨论了移植到CAM上的胚胎乳腺发育阶段依赖的TnT同工型表达。

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