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首页> 外文期刊>Zoological Science >Developmental capacity and chromosome number in the offspring of artificially produced autotetraploids of Rana nigromaculata
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Developmental capacity and chromosome number in the offspring of artificially produced autotetraploids of Rana nigromaculata

机译：人工林蛙的自体四倍体的后代发育能力和染色体数

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A large number of autotetraploids of Rana nigromaculata were produced in order to assess their developmental capacity and chromosome number in their offspring. An original autotetraploid male was first produced by transplanting a nucleus from an embryo (triploid) into an unfertilized egg. Next, eggs were inseminated with sperm of the autotetraploid male, then cold-treated to obtain first-, second-, and third-generation offspring. According to an investigation of the chromosome numbers of the tadpoles by the tail-tip squash method, the three generations of offspring included many tetraploids (50-80%), as well as some diploids, triploids, hexaploids and mosaics at the early tadpole stage. In addition, several percent of the second- and third-generation offspring were found to be aneuploids. Evidently, a complete set of diploid chromosomes was not precisely transferred to all of the next-generation offspring from the sperm of the artificially produced autotetraploid males. These observations suggest that there were some abnormalities in the course of spermatogenesis in the male autotetraploid frogs.
机译：产生了许多黑蛙（Rana nigromaculata）的同源四倍体，以评估其发育能力和后代的染色体数目。首先通过将来自胚胎的核（三倍体）移植到未受精卵中来生产原始的四倍体雄性。接下来，将卵用同四倍体雄性的精子进行授精，然后进行冷处理以获得第一代，第二代和第三代后代。根据尾巴壁球法对the的染色体数目的调查，三代后代包括许多四倍体（50-80％），以及some早期的一些二倍体，三倍体，六倍体和花叶。 。此外，第二代和第三代的后代中有百分之几被发现是非整倍体。显然，没有将一套完整的二倍体染色体精确地转移到人工生产的同源四倍体雄性精子的所有下一代后代中。这些观察结果表明，雄性四倍体青蛙的精子发生过程中存在一些异常。

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