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首页> 外文期刊>Zoological Science >Developmental changes of cardiac and slow skeletal muscle troponin T expression in chicken cardiac and skeletal muscles
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Developmental changes of cardiac and slow skeletal muscle troponin T expression in chicken cardiac and skeletal muscles

机译：鸡心肌和骨骼肌中心肌和慢速骨骼肌肌钙蛋白T表达的发育变化

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摘要

Numerous troponin T (TnT) isoforms are produced by alternative splicing from three genes characteristic of cardiac, fast skeletal, and slow skeletal muscles. Apart from the developmental transition of fast skeletal muscle TnT isoforms, switching of TnT expression during muscle development is poorly understood. In this study, we investigated precisely and comprehensively developmental changes in chicken cardiac and slow skeletal muscle TnT isoforms by two-dimensional gel electrophoresis and immunoblotting with specific antisera. Four major isoforms composed of two each of higher and lower molecular weights were found in cardiac TnT (cTnT). Expression of cTnT changed from high- to low-molecular-weight isoforms during cardiac muscle development. On the other hand, such a transition was not found and only high-molecular-weight isoforms were expressed in the early stages of chicken skeletal muscle development. Two major and three minor isoforms of slow skeletal muscle TnT (sTnT), three of which were newly found in this study, were expressed in chicken skeletal muscles. The major sTnT isoforms were commonly detected throughout development in slow and mixed skeletal muscles, and at developmental stages until hatching-out in fast skeletal muscles. The expression of minor sTnT isoforms varied from muscle to muscle and during development. [References: 39]
机译：肌钙蛋白T（TnT）同工型是通过交替剪接来自心肌，快骨骼肌和慢骨骼肌的三个基因而产生的。除了快速骨骼肌TnT亚型的发育过渡外，在肌肉发育过程中TnT表达的转换还知之甚少。在这项研究中，我们通过二维凝胶电泳和特异性抗血清免疫印迹，准确，全面地研究了鸡心脏和慢速骨骼肌TnT亚型的发育变化。在心脏TnT（cTnT）中发现了四个主要的同工型，分别由较高和较低的两个分子量组成。在心肌发育过程中，cTnT的表达从高分子量亚型改变为低分子量亚型。另一方面，没有发现这种转变，并且在鸡骨骼肌发育的早期仅表达了高分子量同工型。慢骨骼肌TnT（sTnT）的两个主要和三个次要亚型，其中三个在本研究中新发现，在鸡骨骼肌中表达。主要的sTnT亚型通常在发育缓慢的骨骼肌和混合骨骼肌中以及发育阶段直至发育快的骨骼肌中被发现。次要sTnT同工型的表达因肌肉和发育而异。 [参考：39]

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