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首页> 外文期刊>Zoological Science >In vitro cultivation of cells from ovotestis tissue of pigmented Biomphalaria glabrata
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In vitro cultivation of cells from ovotestis tissue of pigmented Biomphalaria glabrata

机译：色素性小球藻的卵睾组织的体外培养

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摘要

Cells derived from ovotestis tissue of pigmented Biomphalaria glabrata, Puerto Rican strain were cultured in double diluted GIT medium supplemented with modification of amino acids components of pigmented B. glabrata, ovotestis and mid-gut region and 3% inactivated fetal calf serum. As a result, two types of cells, epithelial and fibroblastic like cells increased in number during the cultivation. It seem that the medium used in this study is a suitable medium for cultivation of cells from ovotestis of pigemeted B. glabrata. These two types of cells have been maintained by successive transplantation for over 3 passages.
机译：将波多黎各有色素的比目鱼Biomphalaria glabrata卵睾丸组织的细胞培养在双重稀释的GIT培养基中，该培养基补充了有色素的B. glabrata卵，卵睾丸和中肠区域的氨基酸成分以及3％灭活的胎牛血清。结果，在培养期间，两种类型的细胞，上皮细胞和成纤维细胞样细胞的数量增加。似乎在该研究中使用的培养基是一种合适的培养基，用于培养从有斑点的B. glabrata的卵睾丸中提取的细胞。通过连续移植超过3代，可以维持这两种类型的细胞。

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