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首页> 外文期刊>Zoological Science >Cloning of the genes for the pituitary glycoprotein hormone a and follicle-stimulating hormone beta subunits in the Japanese crested ibis, Nipponia nippon
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Cloning of the genes for the pituitary glycoprotein hormone a and follicle-stimulating hormone beta subunits in the Japanese crested ibis, Nipponia nippon

机译：日本朱鹭（Nipponia nippon）垂体糖蛋白激素α和促卵泡激素β亚基基因的克隆

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摘要

We have isolated a part of the gene for the pituitary glycoprotein hormone common a subunit (PGHalpha) and the whole gene for the follicle-stimulating hormone beta subunit (FSHbeta) in the Japanese crested ibis (Nipponia nippon), a critically endangered bird species in East Asia. The nucleotide sequence of a part of the PGHalpha gene (5026 bp) contained three exons holding the whole coding and 3' untranslated regions, but lacked a 5' untranslated region. Its exon-intron structure was similar to that in mammals, but different from that in teleosts in the location of the second intron. For the FSHbeta gene, the nucleotide sequence of 7633 bp was assembled from two phage clones. The exon-intron structure of three exons and two introns was similar to that observed in mammals and teleosts. In the putative promoter region of the ibis FSHbeta gene, a progesterone responsive element (PRE)-like sequence and two AP-1 responsive element-like sequences reported in the ovine FSHbeta gene were not conserved in complete form. The increased number of ATTTA motifs in the putative 3' untranslated region in comparison with those in Japanese quail and chicken FSHbeta cDNA suggested that more rapid degradation of FSHbeta mRNA occurs in this species. Deduced amino acid sequences of the ibis PGHalpha and FSHbeta showed high similarities with those of the corresponding subunits of other avian species. This is the first report on the genomic sequences of the PGHalpha and FSHbeta in an avian species.
机译：我们已经分离了日本朱rest（Nipponia nippon）中的垂体糖蛋白激素常见的一个亚基（PGHalpha）的一部分基因和促卵泡激素β亚基的全部基因（Nipponia nippon），这是日本的一种濒临灭绝的鸟类东亚。 PGHalpha基因的一部分（5026 bp）的核苷酸序列包含三个外显子，它们保留了整个编码区和3'非翻译区，但缺少5'非翻译区。它的外显子-内含子结构与哺乳动物相似，但与第二个内含子所在的硬骨鱼不同。对于FSHbeta基因，从两个噬菌体克隆中组装了7633 bp的核苷酸序列。三个外显子和两个内含子的外显子-内含子结构类似于在哺乳动物和硬骨鱼中观察到的结构。在宜必思FSHbeta基因的推定启动子区域中，绵羊FSHbeta基因中报道的孕酮反应元件（PRE）样序列和两个AP-1反应元件样序列不是完整保守的。与日本鹌鹑和鸡FSHbeta cDNA中的相比，推定的3'非翻译区ATTTA基序数量的增加表明该物种中FSHbeta mRNA的降解速度更快。宜必思PGHalpha和FSHbeta的推导氨基酸序列与其他鸟类的相应亚基具有高度相似性。这是关于禽类中PGHalpha和FSHbeta基因组序列的首次报道。

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