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A transgene and its expression profile are stably transmitted to offspring in transgenic medaka generated by the particle gun method

机译：转基因及其表达谱稳定地传递给通过粒子枪法产生的转基因花aka中的后代

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摘要

A particle gun is used in a potential method for introducing foreign genes into fish. In this paper, we report on the stable transmission of a transgene and its expression profile of the F4 generation in the transgenic medaka (Oryzias latipes). We established four transgenic strains, which contained a green fluorescent protein (GFP) gene controlled by a medaka P-actin promoter, using a particle gun. One more transgenic strain was also generated by microinjection for comparison. In all five strains, the founder was discovered to be mosaic for the transgene. However, from the F1 to F4 generations, transgenes and their expression profiles were stably inherited in the Mendelian manner. The expression profile was common among the five strains regardless of the method for gene transfer: GFP fluorescence became detectable at an early neurula stage. In this stage, the fluorescence was observed ubiquitously in most tissues. As somite developed, GFP fluorescence became intense only in the skeletal muscle and lens but it decreased in other tissues. In adult fish, an intense fluorescence was restricted in the skeletal muscle and lens, while a considerably weak fluorescence was observed in the brain, gill, heart, kidney, spleen, and ovary. From these results, it was concluded that the transgene and its expression profile were stably transmitted to offspring, and thus the particle gun is an effective method for transgenesis in spite of its easiness.
机译：在将外来基因导入鱼类的潜在方法中，使用了粒子枪。在本文中，我们报道了在转基因花aka（Oryzias latipes）中转基因的稳定传播及其F4代的表达情况。我们使用粒子枪建立了四个转基因菌株，其中包含由medaka P-actin启动子控制的绿色荧光蛋白（GFP）基因。还通过显微注射产生了更多的转基因菌株用于比较。在所有五个菌株中，发现创始人都是转基因的花叶。但是，从F1到F4代，转基因及其表达谱以孟德尔方式稳定遗传。无论采用何种基因转移方法，这五种菌株的表达谱都是相同的：GFP荧光在神经元早期就可以检测到。在此阶段，在大多数组织中普遍观察到荧光。随着体节的发育，GFP荧光仅在骨骼肌和晶状体中变强，而在其他组织中下降。在成年鱼中，骨骼肌和晶状体受到强烈的荧光限制，而在脑，腮，心脏，肾脏，脾脏和卵巢中则观察到相当弱的荧光。从这些结果可以得出结论，转基因及其表达谱稳定地传递给后代，因此尽管粒子枪很容易，但它还是一种有效的转基因方法。

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