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首页> 外文期刊>Zoological Science >Changes in the distribution of tenascin and fibronectin in the mouse ovary during folliculogenesis, atresia, corpus luteum formation and luteolysis.
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Changes in the distribution of tenascin and fibronectin in the mouse ovary during folliculogenesis, atresia, corpus luteum formation and luteolysis.

机译：卵泡发生，闭锁，黄体形成和黄体溶解过程中，小鼠卵巢中腱生蛋白和纤连蛋白的分布变化。

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摘要

Tenascin and fibronectin are components of the extracellular matrices that oppose and promote adhesion, respectively. Using immunohistochemical techniques, we studied the distribution of tenascin and fibronectin in the mouse ovary, in which dynamic reconstruction and degeneration occur during folliculogenesis, atresia, ovulation, corpus luteum formation and luteolysis. In growing follicles, tenascin was only detected in the theca externa layer, while fibronectin was detected in the theca externa layer, theca interna layer and basement membrane. During follicular atresia, granulosa cells, which are surrounded by the basement membrane, began to die through apoptosis. In atretic follicles, tenascin was detected in the basement membrane and theca externa layer. Distribution of fibronectin in atretic follicles was similar to that in healthy growing follicles, except that granulosa cells were slightly immunopositive for fibronectin. In young corpus luteum, luteal cells exhibit high 3 beta -hydroxysteroid dehydrogenase (3 beta -HSD) activity, an enzyme indispensable for progesterone production. Tenascin was barely detected in young luteal cells. 3 beta -HSD activity in luteal cells declines with corpus luteum age, and in older corpus luteum there is an increase in apoptotic death of luteal cells. Tenascin was intensely immunopositive in old luteal cells. In contrast, fibronectin immunostaining in luteal cells was relatively constant during corpus luteum formation and luteolysis. Our observations suggest that tenascin is critical in controlling the degenerative changes of tissues in mouse ovaries. Moreover, in all circumstances observed in this study, tenascin always co-localized with fibronectin, suggesting fibronectin is indispensable for the function of tenascin.
机译：腱生蛋白和纤连蛋白分别是对抗和促进粘附的细胞外基质的成分。使用免疫组织化学技术，我们研究了肌腱蛋白和纤连蛋白在小鼠卵巢中的分布，其中在卵泡形成，闭锁，排卵，黄体形成和黄体溶解过程中发生动态重建和变性。在生长的卵泡中，腱生蛋白仅在外膜层中检测到，而纤连蛋白则在外膜层，内膜和基底膜中检测到。在滤泡性闭锁过程中，被基底膜包围的颗粒细胞开始通过凋亡而死亡。在闭锁卵泡中，在基底膜和外膜鞘层中检测到腱生蛋白。纤连蛋白在闭锁卵泡中的分布与健康生长的卵泡中的分布相似，只是颗粒细胞对纤连蛋白的免疫力较弱。在年轻的黄体中，黄体细胞表现出较高的3β-羟类固醇脱氢酶（3β-HSD）活性，这是孕激素生产必不可少的酶。在年轻的黄体细胞中几乎未检测到腱生蛋白。黄体细胞中的3β-HSD活性随着黄体年龄的增长而下降，而在较老的黄体中，黄体细胞的凋亡死亡增加。腱生蛋白在旧的黄体细胞中具有强烈的免疫阳性。相反，在黄体形成和黄体溶解期间，黄体细胞中的纤连蛋白免疫染色相对恒定。我们的观察结果表明，腱生蛋白对于控制小鼠卵巢组织的变性变化至关重要。此外，在本研究中观察到的所有情况下，腱生蛋白总是与纤连蛋白共定位，这表明纤连蛋白对于腱生蛋白的功能是必不可少的。

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