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首页> 外文期刊>Zoological Science >Molecular Cloning and Functional Characterization of Novel Antimicrobial Peptides from the Skin of Brown Frog, Rana zhenhaiensis
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Molecular Cloning and Functional Characterization of Novel Antimicrobial Peptides from the Skin of Brown Frog, Rana zhenhaiensis

机译：镇海蛙棕色蛙皮肤中新型抗菌肽的分子克隆与功能表征

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摘要

Rana zhenhaiensis, a species of brown frog, is widely distributed in central and south China. In the present study, a total of 14 cDNA sequences encoding eight novel antimicrobial peptides (AMPs) were cloned from the synthesized cDNAs of R. zhenhaiensis skin. The eight novel AMPs belong to four families: brevinin-1 (four peptides), brevinin-2 (one peptide), ranatuerin-2 (one peptide) and chensinin-1 (two peptides). five AMPs from the four families (brevinin-1ZHa, brevinin-1ZHb, brevinin-2ZHa, ranatuerin-2ZHa and chensinin-1ZHa) were chemically synthesized, their antimicrobial and hemolytic activities were examined. The results indicated that the five AMPs possess different antimicrobial and hemolytic activities. Of these, brevinin-2ZHa exhibited the strongest and most broad-spectrum antimicrobial activity. Furthermore, scanning electron microscopy (SEM) experiment was carried out to investigate the potential antimicrobial mechanism of chensinin-1ZHa. The result indicated that chensinin-1ZHa may exert its function through disruption of the bacterial membrane.
机译：珍稀林蛙是一种褐蛙，在中国中部和南部广泛分布。在目前的研究中，从合成的R.zhenhaiensis皮肤cDNA克隆出总共14个cDNA序列，编码8个新的抗菌肽（AMP）。八个新型AMP属于四个家族：brevinin-1（四个肽），brevinin-2（一个肽），ranatuerin-2（一个肽）和chensinin-1（两个肽）。化学合成了来自四个家族的五种AMPs（brevinin-1ZHa，brevinin-1ZHb，brevinin-2ZHa，ranatuerin-2ZHa和chensinin-1ZHa），并检测了它们的抗菌和溶血活性。结果表明，这五种AMP具有不同的抗菌和溶血活性。其中，brevinin-2ZHa表现出最强和最广谱的抗菌活性。此外，进行了扫描电子显微镜（SEM）实验，以研究chensinin-1ZHa的潜在抗菌机制。结果表明，chensinin-1ZHa可能通过破坏细菌膜发挥其功能。

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