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首页> 外文期刊>Zoological Science >Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes
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Lectin-Based Profiling of Coelomocytes in Holothuria scabra and Expression of Superoxide Dismutase in Purified Coelomocytes

机译：基于凝集素的整形人鞘膜腔内皮细胞和纯化的内皮细胞中超氧化物歧化酶的表达

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Coelomocytes are the first line of immune defense in marine animals. Their distributions are greatly variable even in the close animal species. In this study, we used lectin staining to aid in the classification and purification of these cells for further investigation of SOD distribution among coelomocytes of H. scraba. We classified coelomocytes into four types: type 1, lymphocytes; type 2, phagocytes; type 3, spherulocytes; and type 4, giant cells. Among four lectins used, Con A appeared to give a broad reactivity against most coelomocytes, except for giant cells. In addition, phagocytes usually engaged the highest fluorescent intensity with most lectins, with the exception of PNA, for which spherulocytes possessed the highest fluorescent intensity. Using FACS for fraction collection, it was found that F1 fraction contained the purest phagocyte population (> 95%), which was highly reactive with anti- superoxide dismutase (SOD) as revealed by immunoblotting and immunofluorescence staining, although some minor staining was also detected in spherulocytes. Our results thus provide a fundamental platform for comparing alterations that may happen to the population and SOD contents of coelomocytes when the sea cucumber is subjected to environmental changes that would activate their immune responses.
机译：腔细胞是海洋动物免疫防御的第一线。即使在近距离的动物物种中，它们的分布也有很大差异。在这项研究中，我们使用凝集素染色来帮助这些细胞的分类和纯化，以进一步研究S. scraba的粒细胞之间的SOD分布。我们将内皮细胞分为四种类型：1型，淋巴细胞，4型和3型。 2型，吞噬细胞; 3型，球细胞;和类型4，巨型细胞在使用的四种凝集素中，除了巨细胞外，Con A似乎对大多数结肠细胞具有广泛的反应性。另外，除了PNA，吞噬细胞通常与大多数凝集素具有最高的荧光强度，而PNA除外，因为球蛋白具有最高的荧光强度。使用FACS进行馏分收集，发现F1馏分包含最纯的吞噬细胞种群（> 95％），与免疫超滤和免疫荧光染色所揭示的抗超氧化物歧化酶（SOD）高度反应，尽管还检测到一些轻微的染色在球细胞中。因此，我们的结果提供了一个基础平台，用于比较当海参受到激活其免疫反应的环境变化时，可能会发生的变化和结肠细胞的SOD含量变化。

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