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首页> 外文期刊>Zoological Science >Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales
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Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales

机译：Medaka秤中成骨细胞和破骨细胞的静态和动态超重力响应

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摘要

Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-kappa B ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain.
机译：鱼鳞是钙化组织的一种形式，类似于在人体骨骼中发现的钙化组织。在medaka规模中，我们同时检测了成骨细胞和破骨细胞，并随后开发了新的规模分析系统。使用该系统，我们通过离心和振动分析了在2、3和4重力（G）载荷下的成骨和破骨反应。加载10分钟后，将离心和振动加载的水垢分别孵育6和24小时，然后测量成骨和破骨活性。通过离心和振动，成骨细胞的活性在2至4 G负荷下显着增加。相反，我们发现在2-G和3-G负荷下，响应于离心和​​振动，破骨细胞活性显着降低。在4-G负荷下，破骨细胞活性在离心作用下也降低，但在4-G负荷下通过振动显着增加，同时成骨细胞活性显着增加。在成骨细胞中表达的破骨细胞活化因子NF-κB配体（RANKL）的受体激活因子的表达在4 G载荷下通过振动显着增加，但在离心载荷下未改变。使用我们的序列分析，在medaka中发现了与骨保护素（OPG）类似的蛋白质序列，称为破骨细胞生成抑制因子。 RANKL / OPG样mRNA的比例在振动负荷量表中明显高于对照量表，尽管离心负荷量表和对照量表之间没有差异。因此，medaka秤提供了一种有用的模型，通过该模型可以分析响应于身体劳损的骨骼代谢。

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