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首页> 外文期刊>Zoological Science >Mitochondrial DNA transcription levels during spermatogenesis and early development in doubly uniparental inheritance of the mitochondrial DNA system of the blue mussel Mytilus galloprovincialis.
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Mitochondrial DNA transcription levels during spermatogenesis and early development in doubly uniparental inheritance of the mitochondrial DNA system of the blue mussel Mytilus galloprovincialis.

机译：蓝贻贝Mytilus galloprovincialis的线粒体DNA系统的双重单亲遗传中，精子发生和早期发育过程中的线粒体DNA转录水平。

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摘要

In some species of bivalve, there are two highly diverged mitochondrial genomes, one found in all individuals (F type) and the other normally in males only (M type). In Mytilus, a maternally-dependent sex ratio of the progeny has been reported. Some females almost exclusively produce daughters, while others produce a high proportion of sons. We previously reported that in M. galloprovincialis, M type mtDNA copy number may be maintained during spermatogenesis and the development of larvae of male-biased mothers to sustain the doubly uniparental inheritance system. In this study, we investigated transcription levels of M type mtDNA before and after fertilization to understand its function in the germ line. First, we quantified transcription levels of M type mtDNA in testicular cells dissected using laser-capture micro-dissection. The transcription levels of M type mtDNA were not significantly different between spermatogonia and spermatocytes versus spermatids and spermatozoa. Next, we examined differences in transcription levels of M type mtDNA between larvae from male-biased and female-biased mothers. The transcription levels of M type mtDNA significantly increased 24 and 48 h after fertilization in male-biased crosses. By contrast, transcription levels significantly decreased in female-biased crosses. These results suggest M type mtDNA may play a role in early germ line formation.
机译：在某些双壳类动物中，线粒体有两个高度不同的基因组，一个在所有个体中均发现（F型），另一个通常仅在雄性中发现（M型）。在Mytilus中，已经报告了后代的母系性别比例。一些女性几乎只生女儿，而另一些则生高比例的儿子。我们以前曾报道过，在加洛斯普罗旺斯菌中，在男性有偏见的母亲的精子发生和幼虫发育期间，可以维持M型mtDNA拷贝数，以维持双亲单亲遗传系统。在这项研究中，我们调查了受精前后M型mtDNA的转录水平，以了解其在种系中的功能。首先，我们定量分析了使用激光捕获显微解剖法解剖的睾丸细胞中M型mtDNA的转录水平。 M型mtDNA的转录水平在精原细胞和精细胞与精子和精子之间没有显着差异。接下来，我们检查了男性偏向和女性偏向的母亲幼虫之间的M型mtDNA转录水平的差异。受精后在雄性偏交杂交中，M型线粒体DNA的转录水平显着提高了24和48 h。相比之下，在女性偏爱的杂交中转录水平显着降低。这些结果表明M型mtDNA可能在早期种系形成中起作用。

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