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首页> 外文期刊>Zoological Science >Individual Identification of Goldfish from Eye Morphology: The Eye Mark Method
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Individual Identification of Goldfish from Eye Morphology: The Eye Mark Method

机译：从眼睛形态学中鉴定金鱼：眼印方法

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摘要

We developed an individual identification method for goldfish based on morphological variation of the iris. Each goldfish has a few dark lines (eye marks) in the rostral and caudal portion of the iris, which are blood vessels underneath the silvery reflective layer. Through the blood vessels and the locally thin reflective layer, the pigment cell layer is partially visible as a dark line. The pattern of the blood vessels was found to be temporally stable and unique to each individual. Using this feature, we successfully identified 10 individual goldfish, each sampled three times within a 4-month time period. The eye mark identification method was confirmed for a further 20 goldfish by comparison with identification based on screening at polymorphic microsatellite loci. The eye mark method is 100% accurate and can be used for studies in which multiple observations of individually identified goldfish are needed over long time periods, to avoid invasive tagging or individual housing that may affect the behavior of the fish.
机译：我们开发了一种基于虹膜形态变化的金鱼个体识别方法。每个金鱼在虹膜的延髓和尾部都有一些黑线（眼线），这些黑线是银色反射层下面的血管。通过血管和局部较薄的反射层，色素细胞层部分可见为黑线。发现血管的模式在时间上是稳定的，并且对于每个人而言都是唯一的。利用此功能，我们成功地识别了10条单独的金鱼，每个金鱼在4个月的时间内采样了3次。通过与基于多态微卫星基因座筛选的鉴定相比较，进一步确定了另外20条金鱼的眼印鉴定方法。眼印方法是100％准确的，可用于需要长时间对个体识别的金鱼进行多次观察的研究，以避免侵入性标签或可能影响鱼类行为的个体饲养。

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