...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zoological Science >Geographic patterns of cytochrome b and Sry gene lineages in the grayred-backed vole Clethrionomys rufocanus from Far East Asia includingSakhalin and Hokkaido
【24h】

Geographic patterns of cytochrome b and Sry gene lineages in the grayred-backed vole Clethrionomys rufocanus from Far East Asia includingSakhalin and Hokkaido

机译：萨哈林和北海道等远东亚洲灰背田鼠rufocanus中的细胞色素b和Sry基因谱系的地理格局

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gray red-backed vole, Clethrionomys rufocanus, from Far East Asia including Sakhalin and Hokkaido is known to harbor intraspecific morphological and cytogenetic variations. Here we analyzed geographic variation in the mitochondrial cytochrome b (Cytb) gene and Y chromosome specific Sry gene by a PCR direct sequencing technique. Determination of sequences in the Cytb gene (1140 bp) in 29 individuals provided 28 haplotypes and construction of a neighbor joining tree clearly indicated that they were grouped into four major lineages, which predominated in Primorskyi territory, Kamchatska-Magadan, Sakhalin, and Hokkaido-Kuril, with 0.02-0.04 sequence divergence (Kimura's distance, all substitutions, all codon positions). The sequences for the Sry region (336-366 bp) included a variable repetitious region of microsatellites such as TG(TC)(2). In total five sequences were recognized which can be grouped into two forms, continental and insular (Sakhalin and Hokkaido) based on the presence or absence of a segment of TG(TC)(3)TG(TC)(4). It was revealed that, therefore, the population of Sakhalin possessed their own type of mitochondrial DNA but the same Sry gene as Hokkaido. A similar trend can be seen in the continental populations. Our results suggested that the population of C. rufocanus in each of the geographic domains accumulated own genetic elements in part but genetic exchanges between neighboring populations occurred during the course of evolution. It is noteworthy that the insular domains, Sakhalin and Hokkaido, have played an important role in raising the amount of genetic diversity in small rodent species.
机译：来自远东亚洲（包括萨哈林岛和北海道）的灰背红田鼠Clethrionomys rufocanus已知具有种内形态和细胞遗传学变异。在这里，我们通过PCR直接测序技术分析了线粒体细胞色素b（Cytb）基因和Y染色体特异性Sry基因的地理变异。确定29位个体的Cytb基因序列（1140 bp）可提供28种单倍型，并构建了一个相邻的连接树，明确表明它们被分为四个主要谱系，主要分布在滨海边疆区，堪察加-马达加丹，萨哈林和北海道-千岛，具有0.02-0.04的序列差异（木村的距离，所有取代，所有密码子位置）。 Sry区的序列（336-366 bp）包括微卫星的可变重复区，例如TG（TC）（2）。根据是否存在TG（TC）（3）TG（TC）（4）片段，总共识别出五种序列，可以将其分为大陆和岛屿两种形式（萨哈林岛和北海道）。因此，据揭示，萨哈林族人拥有自己的线粒体DNA类型，但与北海道拥有相同的Sry基因。在大陆人口中也可以看到类似的趋势。我们的研究结果表明，每个地理区域中的rufocanus种群部分积累了自身的遗传元素，但是相邻种群之间的遗传交换是在进化过程中发生的。值得注意的是，岛屿地区，萨哈林岛和北海道，在提高小型啮齿动物物种的遗传多样性方面发挥了重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号