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首页> 外文期刊>Zoological Science >Analysis of Genetic Diversity in Earthworms Using DNA Markers
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Analysis of Genetic Diversity in Earthworms Using DNA Markers

机译：利用DNA标记分析worm的遗传多样性

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Earthworms are one of the most important and beneficial macrofauna, and are used extensively in organic farming. Earthworms mediate soil biological regulation systems, and produce biogenic structures. They help to maintain soil structure, water infiltration, and regulate the availability of nutrients assimilated by plants. The objectives of this study were to perform morphological and molecular characterizations of 24 earthworm individuals collected from geographically diverse locations to assess the level of genetic variation. For molecular analysis, the effectiveness of RAPD, ISSR, and Universal rice primers (URPs) markers was investigated to identify polymorphism among 24 isolates of earthworms. A total of 62 molecular markers were used for amplification of genomic DNA of earthworms. Of these, 10 RAPD, 10 ISSR, and 10 URPs markers were used for characterization, which showed 95.7%, 96.7% and 98.3% polymorphism, respectively. The dendrogram, generated from the DNA markers by the unweighted pair group method using arithmetic averages, grouped all the isolates into two main clusters. All Eisenia fetida isolates were clustered in group A, whereas group B included three isolates belonging to Eudrilus eugeniae. Molecular markers allowed a rapid assessment of genetic variation among these closely related isolates of earthworms. These results suggest that molecular markers are a good choice for diversity analysis of earthworm individuals.
机译：are是最重要和有益的大型动物之一，广泛用于有机农业。 worm介导土壤生物调节系统，并产生生物结构。它们有助于维持土壤结构，水分渗透并调节植物吸收的养分的利用率。这项研究的目的是对24个从地理上不同的地方收集来的individuals进行形态和分子鉴定，以评估遗传变异的水平。为了进行分子分析，研究了RAPD，ISSR和通用水稻引物（URPs）标记的有效性，以鉴定24种isolate中的多态性。共有62个分子标记被用于扩增of基因组DNA。其中，使用10个RAPD，10个ISSR和10个URPs标记进行表征，其多态性分别为95.7％，96.7％和98.3％。通过使用算术平均值的非加权对法从DNA标记生成的树状图将所有分离物分为两个主要簇。所有的埃森尼亚（Eisenia fetida）分离株都集中在A组中，而B组则包括三个属于Eudrilus eugeniae的分离株。分子标记可以快速评估这些紧密相关的isolate分离株之间的遗传变异。这些结果表明，分子标记是for个体多样性分析的良好选择。

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