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首页> 外文期刊>Zoological Science >Characterization of Gene Expression Endpoints During Postembryonic Development of the NorthernGreen Frog (Rana clamitans melanota)
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Characterization of Gene Expression Endpoints During Postembryonic Development of the NorthernGreen Frog (Rana clamitans melanota)

机译：北方青蛙（Rana clamitans melanota）胚后发育过程中基因表达终点的表征。

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摘要

Postembryonic development of a larval tadpole into a juvenile frog involves the coordinated action of thyroid hormone (TH) across a diversity of tissues. Changes in the frog transcriptome represent a highly sensitive endpoint in the detection of developmental progression, and for the identification of environmental chemical contaminants that possess endocrine disruptive properties. Unfortunately, in contrast with their vital role as sentinels of environmental change, few gene expression tools currently exist for the majority of native North American frog species. We have isolated seven expressed gene sequences from the Northern green frog (Rana clamitans melanota) that encode proteins associated with TH-mediated postembryonic development and global stress response, and established a quantitative real-time polymerase chain reaction (qPCR) assay. We also obtained three additional species-specific gene sequences that functioned in the normalization of the expression data. Alterations in mRNA abundance profiles were identified in up to eight tissues during R. clamitans postembryonic development, and following exogenous administration of TH to premetamorphic tadpoles. Our results characterize tissue distribution and sensitivity to TH of select mRNA of a common North American frog species and support the potential use of this qPCR assay in identification of the presence of chemical agents in aquatic environments that modulate TH action.
机译：幼虫t发育成幼蛙的胚胎后发育涉及跨多种组织的甲状腺激素（TH）的协同作用。青蛙转录组的变化代表了发育进程的检测以及鉴定具有内分泌干扰特性的环境化学污染物的高度敏感的终点。不幸的是，与其作为环境变化哨兵的重要作用相反，目前对于大多数北美本地青蛙物种而言，基因表达工具很少。我们从北方蛙（Rana clamitans melanota）中分离了七个表达的基因序列，这些基因编码与TH介导的胚后发育和整体应激反应相关的蛋白质，并建立了定量实时聚合酶链反应（qPCR）分析方法。我们还获得了三个在表达数据归一化中起作用的其他物种特异性基因序列。 clamitans胚后发育过程中，以及外源施用TH到变态前t后，多达8个组织中均发现了mRNA丰度分布的变化。我们的研究结果表征了常见北美青蛙物种的组织分布和对所选mRNA的TH敏感性，并支持此qPCR分析在鉴定调节TH作用的水生环境中化学试剂的存在中的潜在用途。

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