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Upstream Regulatory Sequences Required for Specific Gene Expression in the Ascidian Neural Tube

机译：海鞘神经管中特定基因表达所需的上游调控序列

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摘要

The relatively simple structure of ascidians and the number of associated molecular resources that are available make ascidians an excellent experimental system for investigating the molecular mechanisms underlying neural tube formation. The ascidian neural tube demonstrates the same basic morphology as that of vertebrates. We have described the expression of the neural tube-specific gene CiNut1, which is expressed within neural tube precursor cells from the gastrula stage, and along the entire length of the neural tube during its formation. In this study, we focused on the transcriptional mechanisms that regulate CiNut1 expression. We found that an approximately 1.0 kb upstream sequence was able to recapitulate endogenous CiNut1 expression. A deletion analysis showed that the 119 bp upstream fragment containing two ZicL-binding consensus sequences and one Fox core sequence could also drive the neural tube-specific expression. When mutations were introduced into the distal ZicL binding site (ZicL1), the neural tube-specific expression almost disappeared. Although the importance of the proximal ZicL site (ZicL2) and the Fox core sequence have yet to be elucidated, we hypothesize that ZicL regulates gene transcription in the entire neural tube of the ascidian.
机译：相对简单的海鞘结构和可用的相关分子资源数量使海鞘成为研究神经管形成的分子机制的优秀实验系统。海鞘神经管显示出与脊椎动物相同的基本形态。我们已经描述了神经管特异性基因CiNut1的表达，该基因在下腹部发育期的神经管前体细胞中以及在其形成期间沿着神经管的整个长度表达。在这项研究中，我们集中于调节CiNut1表达的转录机制。我们发现，大约1.0 kb的上游序列能够概括内源性CiNut1表达。缺失分析显示，包含两个ZicL结合共有序列和一个Fox核心序列的119 bp上游片段也可以驱动神经管特异性表达。当将突变引入远端ZicL结合位点（ZicL1）时，神经管特异性表达几乎消失了。尽管尚未阐明近端ZicL位点（ZicL2）和Fox核心序列的重要性，但我们假设ZicL调节整个海鞘神经管中的基因转录。

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