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首页> 外文期刊>Zoological Science >Molecular Cloning and Expression Profile of Sex-specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres poecilopterus
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Molecular Cloning and Expression Profile of Sex-specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres poecilopterus

机译：性别特异性基因Figla和Dmrt1在原雌性雌雄同体鱼类Halichoeres poecilopterus中的分子克隆和表达谱

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The genes folliculogenesis specific basic helix-loop-helix (facor in the germline alpha, Figla) and doublesex and mab-3 related transcription factor 1 (Dmrt1) are female- and male-specific genes that play key roles in sex differentiation. To obtain a better understanding of the molecular mechanisms underlying female-to-male sex change, we cloned the cDNAs of these genes from an ovary and a testis of the protogynus wrasse, Halichoeres poecilopterus. This fish has two isoforms of Dmrt1, Dmrt1a and Dmrt1b, caused by an alternative splicing. The Dmrt1b has an insertion of three nucleotides (CAG) in the open reading frame. Figla and Dmrt1 displayed gonadal-specific expression and abundant in the ovaries and in the testes, respectively. In particular, levels of Figla expression in the ovaries were higher in the spawning season than in the non-spawning season. Once sex change began, Figla mRNA decreased and Dmrt1 mRNA increased with progression of oocyte degeneration and spermatogenesis. These expression levels were maintained until the completion of the sex change. Low Figla and high Dmrt1 were also observed in testes of primary males, which functioned as a gonochoristic male throughout its life span in this wrasse. The results of this study suggest that these genes may regulate the gonadal transition from ovary to testis by the same mechanism as that of formation and maintenance of the primary testis in H. poecilopterus.
机译：卵泡发生特异的基本螺旋-环-螺旋（种系中的facor，Figla）和doublesex和与mab-3相关的转录因子1（Dmrt1）是雌性和雄性特异性基因，在性别分化中起关键作用。为了更好地了解男女之间发生性别变化的分子机制，我们从Protogynus濑鱼Halichoeres poecilopterus的卵巢和睾丸中克隆了这些基因的cDNA。这条鱼有Dmrt1，Dmrt1a和Dmrt1b的两个同工型，是由另一种剪接引起的。 Dmrt1b在开放阅读框中插入了三个核苷酸（CAG）。 Figla和Dmrt1分别显示出性腺特异性表达，并在卵巢和睾丸中大量表达。特别地，在产卵季节，卵巢中Figla表达水平高于非产卵季节。一旦性别发生变化，随着卵母细胞变性和精子发生的进展，Figla mRNA下降，Dmrt1 mRNA增加。维持这些表达水平直至性别改变完成。在原雄性雄性睾丸中也观察到了低Figla和高Dmrt1，在该濑鱼的整个生命周期中，雄性雄性睾丸功能都是雄性。这项研究的结果表明，这些基因可能通过与poecilopterus初级睾丸的形成和维持相同的机制调节从卵巢到睾丸的性腺过渡。

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