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首页> 外文期刊>Zoological Science >Expression of Membrane Progestin Receptors (mPRs) in Granulosa Cells of Medaka Preovulatory Follicles
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Expression of Membrane Progestin Receptors (mPRs) in Granulosa Cells of Medaka Preovulatory Follicles

机译：膜上孕激素受体（mPRs）在Medaka排卵前卵泡颗粒细胞中的表达。

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Membrane progestin receptor (mPR) alpha on the cell membrane of the oocyte is involved in the meiotic maturation of vertebrates, including teleosts, but little is known about the role of this membrane-bound follicular receptor. We investigated the ovarian expression of membrane progestin receptor (mPR) mRNA in medaka. In follicles that were destined to ovulate, transcripts of mPR alpha and mPR gamma were expressed in the oocytes as well as the granulosa cells. Transcripts of mPR alpha and mPR gamma were expressed at relatively constant levels in the whole ovary and in the preovulatory follicles throughout the 24-h spawning cycle. In vitro incubation of the preovulatory follicles with recombinant medaka luteinizing hormone caused no significant changes in the expression of mPRa alpha and mPR gamma mRNA, suggesting LH-independent follicular expression of these mPR genes. Using HEK293T cells expressing medaka mPRs, forskolin-elevated intracellular cAMP levels were found to be reduced on treatment of the cells with ligand 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP), but only in the cells expressing mPRaa. These results indicate that activation of mPRaa and mPR gamma with DHP may cause differential effects on the granulosa cells. Information obtained from the present study may help to elucidate the role of mPR alpha and mPR gamma in the granulosa cells of the follicles.
机译：卵母细胞细胞膜上的膜孕激素受体（mPR）α与包括硬骨鱼在内的脊椎动物的减数分裂成熟有关，但对该膜结合的卵泡受体的作用知之甚少。我们调查了medaka膜孕激素受体（mPR）mRNA在卵巢中的表达。在预定排卵的卵泡中，卵母细胞以及颗粒细胞中均表达了mPR alpha和mPR gamma的转录本。在整个24小时产卵周期中，mPR alpha和mPR gamma的转录本在整个卵巢和排卵前卵泡中以相对恒定的水平表达。重组花黄体生成素在体外对排卵前卵泡进行孵育不会引起mPRa alpha和mPR gamma mRNA表达的显着变化，表明这些mPR基因的LH非依赖性卵泡表达。使用表达medaka mPR的HEK293T细胞，发现用配体17α，20 beta-dihydroxy-4-pregnen-3-one（DHP）处理细胞后，福司可林升高的细胞内cAMP水平降低，但仅在表达mPRaa。这些结果表明用DHP激活mPRaa和mPRγ可能对颗粒细胞产生不同的影响。从本研究中获得的信息可能有助于阐明mPR alpha和mPR gamma在卵泡颗粒细胞中的作用。

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