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首页> 外文期刊>Zeitschrift fur Naturforschung, C. A Journal of Biosciences >Hydrogen peroxide from the oxidative burst is not involved in the induction of taxol biosynthesis in Taxus chinensis cells
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Hydrogen peroxide from the oxidative burst is not involved in the induction of taxol biosynthesis in Taxus chinensis cells

机译：来自氧化爆发的过氧化氢不参与红豆杉细胞中紫杉醇的生物合成诱导

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摘要

In cell suspension cultures of Taxus chinensis, 40 mg/l fungal elicitor from Aspergillus niger and 20 mum HgCl2 elicited 5.7 and 3.6 mg/1 taxol, which was a 9-fold and 5-fold increase vs. compared with the control, respectively. The fungal elicitor induced hydrogen peroxide (H2O2) accumulation but HgCl2 did not, indicating that H2O2 was not necessary for enhancement of taxol induced by elicitor. Compared with the treatment with fungal elicitor alone, exogenous catalase, ascorbic acid, diphenylene iodonium and superoxide dismutase induced a 0.45, 0.4, 0.7 and 1.4-fold H2O2, but elicited taxol production, which was 0.98, 1.2, 1.1 and 0.9-fold, respectively, vs. non-treated cells. Elicitor-induced taxol production was not accorded with the amount of H2O2 production. [References: 12]
机译：在红豆杉的细胞悬浮培养物中，来自黑曲霉的40 mg / l真菌引发剂和20 mHgCl2引起5.7和3.6 mg / 1紫杉醇，分别比对照增加9倍和5倍。真菌引发剂诱导过氧化氢（H2O2）积累，但HgCl2却没有，表明H2O2对于增强由引发剂诱导的紫杉醇不是必需的。与单独使用真菌引发剂相比，外源过氧化氢酶，抗坏血酸，二亚苯基碘鎓和超氧化物歧化酶诱导产生的H2O2含量分别为0.45、0.4、0.7和1.4倍，但紫杉醇的产量分别为0.98、1.2、1.1和0.9倍，相对于未处理的细胞激发子诱导的紫杉醇产量与H2O2产量不符。 [参考：12]

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