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首页> 外文期刊>Vaccine >Immunogenicity of plasmid DNA encoding the 62 kDa fragment of Schistosoma japonicum myosin.
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Immunogenicity of plasmid DNA encoding the 62 kDa fragment of Schistosoma japonicum myosin.

机译：编码日本血吸虫肌球蛋白62 kDa片段的质粒DNA的免疫原性。

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摘要

The recombinant Schistosoma mansoni 62 kDa myosin fragment (rIrV-5) is highly protective in experimental animals. However, vaccination of mice and rats with the recombinant S. japonicum homologue (rSj62) did not induce significant resistance againstS. japonicum infection. To explore alternative ways of presenting this antigen, a plasmid vector (VRSj62) was constructed which encodes Sj62 using the VR1020 vector and tested. Four immunizations with 10μg VRSj62 DNA alone were sufficient to induce high and progressively increasing levels of IgG antibodies against rSj62 with increasing numbers of injections in CBA/Ca mice (IgG titre >=1:25000), and 3 injections with 50μg VRSj62 DNA alone induced significant IgG responses in C57B1/6 mice (IgG titre, 1:1600). Vaccination with plasmid DNA entrapped in cationic liposomes or together with pUC19 DNA as a source of CpG motifs, both of which have been reported to enhance immune responses, did not enhance specific antibody production. In spite of the stimulation of specific antibodies against rSj62 with the naked DNA construct, no resistance to challenge was demonstrated.
机译：重组曼氏血吸虫62 kDa肌球蛋白片段（rIrV-5）在实验动物中具有高度保护性。然而，用重组日本血吸虫同源物（rSj62）对小鼠和大鼠进行疫苗接种不会诱导对沙门氏菌的显着抗性。日本血吸虫感染。为了探索呈递该抗原的替代方式，构建了质粒载体（VRSj62），其使用VR1020载体编码Sj62，并进行了测试。在CBA / Ca小鼠中注射次数增加（IgG滴度> = 1：25000），仅用10μgVRSj62 DNA进行的四次免疫足以诱导高水平和逐步增加的针对rSj62的IgG抗体（IgG滴度> = 1：25000），并且仅用3μgVRSj62 DNA诱导在C57B1 / 6小鼠中产生明显的IgG反应（IgG滴度，1：1600）。用阳离子脂质体中包裹的质粒DNA或与pUC19 DNA一起作为CpG基序的来源进行疫苗接种，这两种方法均已报道可增强免疫应答，但并未提高特异性抗体的产生。尽管用裸露的DNA构建体刺激了针对rSj62的特异性抗体，但未显示出对攻击的抗性。

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